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PsaE/PSI-E subunit of photosystem I Polyclonal Antibody
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PsaE/PSI-E subunit of photosystem I Antibody (AS08-324A) in WB
Thylakoid membranes (10 µg of total chlorophyll) extracted freshly from Hordeum vulgare leaves with 100 m M HEPES-KOH (p H 7.5), 0.3 M sorbitol, 2 m M EDTA, and 1 m M Mg Cl 2 and denatured with a LaemmLi buffer at 80°C for 5 min were separated on 12% SDS-PAGE and blotted 1 h to nitrocellulose (pore size of 0.2 um), using semi-dry transfer. Blot was blocked with 4% milk for 2 h/RT with agitation. Blot was in... View More
产品信息
AS08-324A
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated synthetic peptide derived from PsaE N-terminal part.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana, Hordeum vulgare
靶标信息
psaE encodes the photosystem I reaction center subunit E, a small, basic, peripheral protein that localizes to the stromal/cytoplasmic (reducing) side of the photosystem I complex where soluble electron carriers bind. Structurally, PsaE adopts an SH3-like fold dominated by a compact five-stranded beta-sheet core connected by flexible surface loops, consistent with a role as an exposed interaction module rather than a membrane-embedded component. Functionally, PsaE contributes to forming and tuning the electrostatic docking surface for the soluble acceptors ferredoxin and flavodoxin, thereby optimizing productive binding and electron transfer from the terminal photosystem I acceptors; genetic loss of psaE can markedly weaken ferredoxin association/photoreduction efficiency and is associated with increased susceptibility to oxygen-dependent reactive oxygen species formation, indicating that PsaE helps maintain efficient acceptor-side electron flow and limits adventitious oxygen reduction under illumination.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AT2G20260; Photosystem I reaction center subunit E; photosystem I reaction center subunit IV; photosystem I reaction center subunit IV B; photosystem I subunit E; photosystem I subunit IV; PS I subunit E; PsaE protein; psaE1; psaE2; PSI-E
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