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PsaG/PSI-G subunit of photosystem I Polyclonal Antibody
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PsaG/PSI-G subunit of photosystem I Antibody (AS20-4368) in WB
4 µg/well of total chlorophyll from frozen thylakoids extracted freshly from Arabidopsis thalianaas described in Bassi et al., 1985 were separated on 4-15% SDS-PAGE and blotted 1 h to PVDF semi-dry. Blot was blocked with 5% milk for 1 h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1:1,000 in TBS-T ON/4°C with agitation. The antibody solution was decanted and the blot was ri... View More
产品信息
AS20-4368
产品规格
种属反应
Plant
宿主/亚型
Chicken
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated peptide derived from Arabidopsis thaliana PsaG.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
PSAG encodes the chloroplast-localized photosystem I reaction center subunit V (PSI-G), a small thylakoid membrane protein that is synthesized with an N-terminal chloroplast transit peptide and then integrated into the photosystem I (PSI) complex. In Arabidopsis thaliana, the mature protein is ~160 amino acids (~17 kDa) and is strongly hydrophobic, consistent with multiple transmembrane helices that anchor it in the thylakoid membrane within PSI-LHCI supercomplexes. Functional genetic analyses indicate PSI-G is not absolutely essential for photoautotrophic growth under standard conditions, but loss of PSAG perturbs PSI polypeptide composition and antenna connectivity, supporting a role for PSI-G in stabilizing the PSI core and maintaining proper association/organization of peripheral light-harvesting components; these effects can influence photosynthetic electron flow and related regulatory phenomena such as excitation energy distribution between photosystems.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: photosystem I subunit G; PSI-G
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