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Agrisera
PsbE/Cytochrome b559 subunit alpha (Algal) Polyclonal Antibody
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PsbE/Cytochrome b559 subunit alpha (Algal) Antibody (AS19-4330) in WB
Samples: 1 - 10 µg of Synechocystis PCC 6,803 isolated thylakoid membranes 2 - 20 µg of Synechocystis PCC 6,803 isolated thylakoid membranes 3 - 10 µg of Psb E deletion mutant Synechocystis PCC 6,803 isolated thylakoid membranes 10 or 20 µg/well of total protein from isolated thylakoid membranes with MES buffer (25 m M MES/Na OH, p H 6.5,10 m M Mg Cl 2,10 m M Ca Cl 2, and 25% glycerol) and denatured with SD... View More
产品信息
AS19-4330
产品规格
种属反应
Bacteria
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated peptide derived from algal PsbE sequences, including Chlamydomonas reinhardtii PsbE.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Synechocystis PCC 6803
靶标信息
psbE is a plastid-encoded gene in oxygenic photosynthetic organisms (including chloroplasts of plants and cyanobacteria) that produces a very small integral thylakoid-membrane protein forming the alpha subunit of cytochrome b559 within photosystem II (PSII). The PsbE polypeptide is typically ~80-90 amino acids long and is dominated by a single membrane-spanning alpha helix; together with the PSII cytochrome b559 beta subunit, it binds a b-type heme cofactor via a conserved histidine axial ligand contributed by each subunit, creating a tightly associated cytochrome embedded in the PSII reaction-center core. Functionally, cytochrome b559 is required for normal PSII activity and stability: genetic disruption of psbE (often affecting the psbE/psbF unit) or mutation of the heme-ligating histidine abolishes PSII electron transfer, supporting an essential role in PSII assembly and/or maintenance, with additional evidence that cytochrome b559 participates in protective redox pathways that help limit photodamage during stress conditions.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: apocytochrome b559 alpha subunit; cytochrome b559 subunit alpha; photosystem II cytochrome b559 alpha subunit; photosystem II protein V; PSII reaction center subunit V
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