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Agrisera
PsbO/Photosystem II manganese-stabilizing polypeptide (algal) Polyclonal Antibody
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PsbO/Photosystem II manganese-stabilizing polypeptide (algal) Antibody (AS21-4689) in WB
10 µg/well of total protein extracted from Chlamydomonas reinhardtii, total cell extract were denatured with Invitrogen LDS sample buffer (4 X) at 70°C/5 min. and separated on Invitrogen Nu PAGE Bis-Tris 4-12% SDS-PAGE 45 min and blotted for 1 h to Invitrogen PVDF (pore size of 0.45 µm), using: wet transfer. Blot was blocked with 5% milk in TBS-T for: 1 h/RT with agitation, and incubated in the primary anti... View More
产品信息
AS21-4689
产品规格
种属反应
Algae
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated peptide derived from Chlamydomonas reinhardtii PsbO protein sequence.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Chlamydomonas reinhardtii
靶标信息
psbO encodes the oxygen-evolving enhancer protein 1 (PsbO), a soluble extrinsic subunit of photosystem II that is targeted to chloroplasts and localizes on the lumenal face of the thylakoid membrane as part of the oxygen-evolving complex. PsbO is synthesized as a precursor bearing an N-terminal targeting peptide that is removed during import, yielding a mature ~33 kDa lumenal protein. Structurally, PsbO is dominated by a beta-barrel framework with prominent loop regions (often described as a flexible "head" domain) and contains a conserved disulfide bond that contributes to protein stability. Functionally, PsbO is broadly conserved across oxygenic photosynthetic organisms and is required to support efficient light-driven water oxidation by stabilizing and shielding the Mn4CaO5 catalytic cluster and helping maintain the proper ionic environment for oxygen evolution; it interacts with lumen-exposed regions of core photosystem II subunits (including D1, D2, CP43, and CP47) and contributes to overall oxygen-evolving complex integrity and activity.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 33 KDA OXYGEN EVOLVING POLYPEPTIDE 1; 33 KDA SUBUNIT OF OXYGEN EVOLVING SYSTEM OF PHOTOSYSTEM II; MANGANESE-STABILIZING PROTEIN 1; MSP-1; OE33; OEC 33 KDA SUBUNIT; OEC33; OEE1; OEE33; OXYGEN EVOLVING COMPLEX 33 KILODALTON PROTEIN; OXYGEN EVOLVING COMPLEX SUBUNIT 33 KDA; OXYGEN EVOLVING ENHANCER PROTEIN 33; oxygen-evolving complex psbo; PSBO; PSBO-1; PSBO1
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