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PsbO1/33 kDa of the oxygen evolving complex (OEC) of PSII Polyclonal Antibody
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PsbO1/33 kDa of the oxygen evolving complex (OEC) of PSII Antibody (AS14-2824) in WB
30 µg of thylakoid protein from Arabidopsis thaliana Columbia-0 extracted with 330 m M sorbitol, 50 m M Hepes-KOH (p H 7.8) were separated on 15% SDS-PAGE and blotted 1 h to PVDF using semi-dry transfer. Blots were blocked with 5% milk for 1 h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:1,000 for 6 h at 4°C with agitation. The antibody solution was ... View More
产品信息
AS14-2824
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated peptide chosen from Arabidopsis thaliana PsbO1.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
保存液
whole serum
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
PSBO1 encodes a soluble, nuclear-encoded extrinsic photosystem II (PSII) lumenal protein that is targeted to chloroplasts and accumulates on the lumen-facing side of thylakoid membranes as a core component of the oxygen-evolving complex. The gene produces a precursor polypeptide that is processed after import, yielding a mature protein of ~33 kDa that belongs to the conserved PsbO/manganese-stabilizing protein family and lacks transmembrane helices, consistent with peripheral association to PSII rather than membrane integration. Functionally, PsbO1 is the major PsbO isoform in Arabidopsis and is required for efficient water-splitting: it stabilizes the catalytic Mn4CaO5 cluster and supports proper oxygen evolution, with loss-of-function mutants showing impaired PSII performance and defects in oxygen-evolving complex stability and Ca utilization.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 33 KDA OXYGEN EVOLVING POLYPEPTIDE 1; AT5G66570; K1F13.25; K1F13_25; MANGANESE-STABILIZING PROTEIN 1; MSP-1; OE33; OEC33; OEE1; OEE33; OXYGEN EVOLVING COMPLEX 33 KILODALTON PROTEIN; OXYGEN EVOLVING COMPLEX SUBUNIT 33 KDA; OXYGEN EVOLVING ENHANCER PROTEIN 33; PS II oxygen-evolving complex 1; PSBO; PSBO-1; PSBO1
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