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PsbO2/33 kDa of the oxygen evolving complex (OEC) of PSII Polyclonal Antibody
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PsbO2/33 kDa of the oxygen evolving complex (OEC) of PSII Antibody (AS14-2825) in WB
30 µg of thylakoid protein from Arabidopsis thaliana Columbia-0 extracted with 330 m M sorbitol, 50 m M Hepes-KOH (p H 7.8) were separated on 15% SDS-PAGE and blotted 1 h to PVDF using semi-dry transfer. Blots were blocked with 5% milk for 1 h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:1,000 for 6 h at 4°C with agitation. The antibody solution was ... View More
产品信息
AS14-2825
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated peptide chosen from Arabidopsis thaliana PsbO2.
偶联物
形式
Lyophilized
保存液
whole serum
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
PSBO2 encodes a nuclear-encoded, chloroplast-targeted extrinsic subunit of photosystem II that accumulates on the luminal side of the thylakoid membrane as part of the oxygen-evolving complex. The gene produces an mRNA that is translated as a precursor polypeptide with an N-terminal chloroplast transit peptide that is removed upon import, yielding a soluble, predominantly beta-strand-rich lumenal protein of the conserved PsbO/manganese-stabilizing protein family that associates non-covalently with the PSII core rather than spanning the membrane. Functionally, PsbO2 contributes to efficient oxygen evolution by stabilizing the water-oxidizing Mn4CaO5 catalytic cluster and helping maintain the proper ionic and structural environment required for water splitting; in Arabidopsis, genetic analyses indicate that PsbO2 is the minor isoform relative to PsbO1 yet is important for PSII performance under light stress and has a specific role in regulating phosphorylation state and turnover/repair of the PSII reaction-center D1 protein, consistent with a contribution to PSII maintenance in addition to catalysis support.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AT3G50820; F18B3.100; MSP-2; OEC33; PSBO; PSBO-2
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