Invitrogen

QA-1b Monoclonal Antibody (6A8.6F10.1A6)

Name: QA-1b Monoclonal Antibody (6A8.6F10.1A6)
Brand: Invitrogen
SKU: MA5-24801

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (3)

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QA-1b Antibody (MA5-24801) in WB

Western blot analysis of QA-1b in mouse thymus tissue lysate. Samples were incubated with QA-1b monoclonal antibody (Product # MA5-24801) using a dilution of 2 µg/mL.

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产品信息

MA5-24801

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

流式细胞分析 (Flow)

1:10-1:1,000

免疫沉淀 (IP)

Assay-Dependent

产品规格

种属反应

Human, Mouse

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

6A8.6F10.1A6

抗原

Synthetic peptide from the alpha-2 domain of Qa-1b.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2717274

靶标信息

The Qa-1b gene is the mouse homolog of the human HLA-E, a non-classical major histocompatibility complex class Ib protein encoded by the T23 gene within the T region of the H-2 complex. Qa-1b plays a crucial role in immune regulation by serving as a ligand for murine CD94/NKG2A natural killer (NK) cell inhibitory receptors. It primarily binds to a nonameric peptide known as the Qa-1 determinant modifier (Qdm), derived from the leader sequence of other MHC class Ia molecules. In mouse melanoma cells, particularly the B16-F10 line, Qa-1b is overexpressed, and its knockdown has been observed to inhibit cell proliferation and migration, while also promoting cell cycle arrest at the G0/G1 phase and facilitating apoptosis via the Ras-Raf-MAPK signaling pathway. As such, Qa-1b serves as an oncogenic factor in melanoma models, offering potential therapeutic pathways for cancer treatment. Its non-polymorphic nature contributes to its conserved function across various mouse strains, with only two dominant allelic variants, Qa-1a and Qa-1b. Despite sharing a 73% homology with human HLA-E, differences in peptide repertoires and binding affinities highlight its unique role in murine immune responses.

仅用于科研。不用于诊断过程。未经明确授权不得转售。