Agrisera

RA/Rubisco activase Polyclonal Antibody

Name: RA\/Rubisco activase Polyclonal Antibody
Brand: Agrisera
SKU: AS22-4868

查看其他3个Rubisco activase抗体

说明书

证书

实验方案

问题与答案

技术支持

说明书

证书

实验方案

问题与答案

技术支持

说明书

证书

实验方案

问题与答案

技术支持

Additional Information:

抗体检测数据 (2)

RA/Rubisco activase Antibody in Western Blot (WB)

图: 1 / 2

RA/Rubisco activase Antibody (AS22-4868) in WB

Samples from left to right: 1 – MW marker 2 – 10 µg of Arabidopsis thaliana whole leaf extract 3 – 10 µg of Hordeum vulgare whole leaf extract 4 – 10 µg Chlamydomonas reinhardtii whole cell extract 10 µg/well of respective cell extract was denatured with Invitrogen LDS sample buffer (4 X) at 70°C/5 min. Samples were separated on Invitrogen Nu PAGE Bis-Tris 4-12% SDS-PAGE 45 min and blotted for 1 h to Invitrogen PVDF (pore size of 0.45 um), using: wet transfer. Blot was blocked with 5% milk in TBS-T for: 1 h/RT with agitation. Blot was incu... View More

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产品信息

AS22-4868

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:5,000

产品规格

种属反应

Algae, Plant

宿主/亚型

Rabbit

分类

Polyclonal

类型

Antibody

抗原

Purified, recombinant Rubisco activase from Gossypium hirsutum.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

50 µg

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4

内含物

no preservative

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

For reconstitution add 50 µL of sterile water.

Specific Species Reactivity: Arabidopsis thaliana, Chlamydomonas reinhardtii, Hordeum vulgare

靶标信息

Ribulose bisphosphate carboxylase/oxygenase activase is an enzyme localized to chloroplasts which activates Rubisco by promoting ATP-dependent conformational changes. Alternative name: RuBisCo activase RCA. There are two forms of activase (alpha and beta) in some species (for example Arabidopsis, camelina, spinach, rice) and only one form in other species (tobacco, maize,Chlamydomonas). Alpha is about 46-47 kDa, beta is about 42 kDa. Species that have only one form have the beta form.

仅用于科研。不用于诊断过程。未经明确授权不得转售。