Invitrogen

RAB10 Polyclonal Antibody

Name: RAB10 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-79901

高级验证

查看其他8个RAB10抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (4)
高级验证 (1)

RAB10 Antibody in Immunocytochemistry (ICC/IF)

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RAB10 Antibody (PA5-79901) in ICC/IF

Immunofluorescence analysis of RAB10 was performed using 70% confluent log phase SK-O-V3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with RAB10 Polyclonal Antibody (Product # PA5-79901) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488 (Product # A32790), (1:2... View More

Product Image Gallery

RAB10 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. SK-O-V3 cells were transfected with RAB10 siRNA and decrease in signal intensity was observed in Western Blot application using RAB10 Polyclonal Antibody (Product # PA5-79901). {KD}

Immunogen image

Immunogen Image RAB10 Polyclonal Antibody

产品信息

PA5-79901

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

免疫细胞化学 (ICC/IF)

1:100

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthetic peptide corresponding to a sequence at the C-terminus of human RAB10 (153-185aa AKANINIEKAFLTLAEDILRKTPVKEPNSENVD).

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 5mg BSA

内含物

0.05mg sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2747016

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: human MCF-7 whole cell, human Hela whole cell, human A549 whole cell, rat brain tissue, rat C6 whole cell, mouse brain tissue, mouse NIH/3T3 whole cell.

靶标信息

RAB10 belongs to the RAS superfamily of small GTPases. RAB proteins localize to exocytic and endocytic compartments and regulate intracellular vesicle trafficking.

仅用于科研。不用于诊断过程。未经明确授权不得转售。