Invitrogen

RAB11A Monoclonal Antibody (4H9)

Name: RAB11A Monoclonal Antibody (4H9)
Brand: Invitrogen
SKU: MA5-49197

查看其他11个RAB11A抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (11)

RAB11A Antibody in Immunocytochemistry (ICC/IF)

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RAB11A Antibody (MA5-49197) in ICC/IF

Immunocytochemistry analysis of RAB11A in T-47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in RAB11A Monoclonal antibody (Product # MA5-49197) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate ... View More

RAB11A Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RAB11A Antibody in Flow Cytometry (Flow)

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Immunogen image

Immunogen Image RAB11A Monoclonal Antibody (4H9)

产品信息

MA5-49197

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.25-0.5 µg/mL

免疫组化（石蜡） (IHC (P))

2-5 µg/mL

免疫细胞化学 (ICC/IF)

5 µg/mL

流式细胞分析 (Flow)

1-3 µg/1x10^6 cells

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Mouse / IgG2b

分类

Monoclonal

类型

Antibody

克隆号

4H9

抗原

A synthetic peptide corresponding to a sequence at the C-terminus of human Rab11A (171-211aa EIYRIVSQKQMSDRRENDMSPSNNVVPIHVPPTTENKPKVQ).

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 4mg trehalose

内含物

no preservative

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_3074855

产品详细信息

Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

Immunogen sequence is identical to the related mouse and rat sequences.

Positive Control - WB: human 293T whole cell, human SH-SY5Y whole cell, human MCF-7 whole cell, human Hela whole cell, rat brain tissue, rat PC-12 whole cell, mouse brain tissue, mouse Neuro-2a whole cell. IHC: human spleen tissue, human gastric carcinoma tissue, human placenta tissue. ICC/IF: T-47D cell. Flow: ANA-1 cell, RH35 cell, U937 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

靶标信息

Rab11A is a member of the YPT-1 subfamily of Ras-related GTP-binding proteins. Rab11A mRNA has been detected in a variety of tissues including brain, testis, spleen, heart, and gastrointestinal mucosa. In the gastric fundus the Rab11A protein is expressed in parietal cell tubulovesicles membranes. Expression of the Rab11A protein appears to occur in discrete epithelial cell populations where it localizes to apical vesicular populations. Recent studies have shown Rab11A is essential for resecretion of alpha-synuclein out of neuronal cells via endosome recycling and is required for exocytosis of discoidal/fusiform vesicles of bladder umbrella cells. Rab11A is also implicated in biogenesis of Birbeck granules and endosomal activation of the PI3K/AKT signaling pathway via G beta gamma trafficking.

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