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RAB27A Antibody (PA5-79904) in IHC (P)
Immunohistochemistry analysis of RAB27A on paraffin-embedded human mammary cancer tissue. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with RAB27A polyclonal antibody (Product# PA5-79904) with a dilution of 1 µg/mL (overnight at 4°C), and followed by biotinylated goat anti-rabbit IgG (30 minutes at 37°C... View More
产品信息
PA5-79904
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
C. elegans
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E. coli-derived human RAB27A recombinant protein (Position: L98-K216).
偶联物
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 4mg trehalose
内含物
0.05mg sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human Jurkat whole cell, human MCF-7 whole cell, human HepG2 whole cell, human A549 whole cell, rat stomach tissue, rat thymus tissue, mouse thymus tissue, mouse NIH3T3 whole cell. IHC: human lung cancer tissue, human mammary cancer tissue, rat small intestine tissue, rat spleen tissue, mouse spleen tissue. Flow: K562 cell.
靶标信息
The protein encoded by this gene belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Mutations in this gene are associated with Griscelli syndrome type 2. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: ashen; GTP-binding protein Ram; low Mr GTP-binding protein; mutant Ras-related protein Rab-27A; Rab-27; Ram p25; Ras-related protein Rab-27A
基因别名: 2210402C08Rik; 2410003M20Rik; 4933437C11Rik; ash; GS2; HsT18676; RAB27; RAB27A; RAM
UniProt ID: (Human) P51159, (Mouse) Q9ERI2, (Rat) P23640
Entrez Gene ID: (Human) 5873, (Mouse) 11891, (Rat) 50645
protein targeting
exocytosis
small GTPase mediated signal transduction
blood coagulation
positive regulation of gene expression
antigen processing and presentation
melanocyte differentiation
melanosome localization
melanosome transport
multivesicular body organization
cytotoxic T cell degranulation
natural killer cell degranulation
positive regulation of exocytosis
synaptic vesicle transport
positive regulation of phagocytosis
multivesicular body sorting pathway
complement-dependent cytotoxicity
positive regulation of regulated secretory pathway
positive regulation of reactive oxygen species biosynthetic process
positive regulation of constitutive secretory pathway
exosomal secretion
vesicle docking involved in exocytosis
protein secretion
protein transport
vesicle-mediated transport
Rab protein signal transduction
pigmentation
pigment granule localization
pigment granule transport
intracellular protein transport
metabolic process
