Invitrogen

RAB3d Polyclonal Antibody

Name: RAB3d Polyclonal Antibody
Brand: Invitrogen
SKU: PA1-773
Price: 4899.00 CNY
Availability: InStock

3 篇参考文献

查看其他8个RAB3d抗体

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

Additional Information:

抗体检测数据 (1)

RAB3d Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 1

RAB3d Antibody (PA1-773) in ICC/IF

Immunofluorescence analysis of RAB3D was performed using 70% confluent log phase 3T3-L1 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with RAB3D Rabbit Polyclonal Antibody (Product # PA1-773) at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A... View More

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

Immunogen image

Immunogen Image RAB3d Polyclonal Antibody

产品信息

PA1-773

应用

建议稀释比

已发表文章

免疫印迹 (WB)

2.0 µg/mL

查看1篇已发表文章 1篇已发表文章

免疫组化 (IHC)

查看2篇已发表文章 2篇已发表文章

免疫细胞化学 (ICC/IF)

2 µg/mL

产品规格

种属反应

Mouse, Rat

已发表种属

Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to residues M(1) A S A S E P P A S P R D A A D Q N(18) of rat RAB3D.

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

100 µg

纯化类型

Protein A

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

Ambient (domestic); Wet ice (international)

AB_2177236

产品详细信息

PA1-773 detects recombinant rat and mouse rab 3D. This antibody does not detect rab 3A, 3B, or 3C.

PA1-773 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~29 kDa protein representing recombinant mouse rab 3D.

PA1-773 immunogen is a synthetic peptide corresponding to residues M(1) A S A S E P P A S P R D A A D Q N(18) of rat RAB3D. This sequence is completely conserved in mouse. This immunizing peptide (Cat. # PEP-144) is available for use in neutralization and control experiments.

靶标信息

Rab proteins are low-molecular-weight GTP-binding proteins that form the largest branch of the Ras superfamily of GTPases. Located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Three membrane proteins, synaptosomal associated protein of 25 kDa (SNAP-25), synaptobrevin, and syntaxin, form the core of a ubiquitous membrane fusion machine that interacts with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and a-SNAP. Rab proteins, in coordination with the core fusion machinery and Munc-18, help to mediate vesicle docking and fusion. There exist over 40 rab proteins that have been described in mammals. Rab 3D has been shown to be present, in part, in the tongue, pancreas, and liver.

仅用于科研。不用于诊断过程。未经明确授权不得转售。