Invitrogen

RAB9 Monoclonal Antibody (Mab9)

Name: RAB9 Monoclonal Antibody (Mab9)
Brand: Invitrogen
SKU: MA3-067

高级验证

4个已发表图片

19 篇参考文献

查看其他17个RAB9抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

RAB9 Antibody in Immunocytochemistry (ICC/IF)

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RAB9 Antibody (MA3-067) in ICC/IF

Immunofluorescence analysis of RAB9 was performed using HEK-293 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with RAB9 Mouse Monoclonal Antibody (Product # MA3-067) at 1:200 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32723) at a dilution of 1:2000 for... View More

RAB9 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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RAB9 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HEK-293 cells were transfected with RAB9A siRNA and reduction of signal was observed in Western Blot using RAB9 Mouse Monoclonal Antibody (Product # MA3-067). {KD}

产品信息

MA3-067

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:5,000

查看5篇已发表文章 5篇已发表文章

免疫组化 (IHC)

查看5篇已发表文章 5篇已发表文章

免疫组化（石蜡） (IHC (P))

1:20

免疫细胞化学 (ICC/IF)

1:200-1:500

查看8篇已发表文章 8篇已发表文章

流式细胞分析 (Flow)

查看1篇已发表文章 1篇已发表文章

免疫沉淀 (IP)

Assay-dependent

免疫电镜技术 (IM)

Assay-dependent

产品规格

种属反应

Bovine, Dog, Hamster, Human, Non-human primate, Rat

已发表种属

C. elegans, Hamster, Human, Mouse

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

Mab9

抗原

Recombinant canine rab 9a.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2175599

产品详细信息

MA3-067 recognizes prenylated and non-prenylated rab 9 from canine, human, monkey, rat, hamster and bovine tissues. This antibody does not cross-react with other rab family members.

MA3-067 has been successfully used in Western blot, immunofluorescence, EM immunocytochemistry and immunoprecipitation procedures. By Western blot, this antibody detects an ~26 kDa protein representing rab 9 from K562 cell extract.

The MA3-067 antigen is recombinant canine rab 9a.

靶标信息

Rab proteins are a family of Ras-like GTPases involved in intracellular compartment protein transport. Different members of the 40+ member rab family are responsible for docking and fusion of transport vesicles between different compartments within the cell. Rab 9 is required for trafficking mannose 6-phosphate receptor between the late endosome to trans-Golgi network (TGN). By facilitating receptor transport, rab 9 enables cells to efficiently recycle important cellular trafficking components. It is functionally necessary for rab 9, like other rab family members, to be prenylated by two 20-carbon geranylgeranyl groups at the C-terminus. Most prenylated rab 9 is membrane bound, however, 10-20% of the cellular pool of rab 9 is bound to GDP dissociation inhibitor-alpha (GDI-alpha) in the cytosol. GDI recycles prenylated, GDP bound rab 9 from their fusion targets back to their membranes of origin.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: rab-9A; rab9; rab9 GTP-binding protein; RAB9, member RAS oncogene family; RAB9-like protein; RAB9A, member RAS oncogene family; Ras-related protein Rab-9A

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基因别名: RAB9; RAB9A

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UniProt ID: (Human) P51151, (Dog) P24408, (Rat) Q99P75