Invitrogen

RAC1 Polyclonal Antibody

Name: RAC1 Polyclonal Antibody
Brand: Invitrogen
SKU: PA1-091

高级验证

10个已发表图片

27 篇参考文献

查看其他11个RAC1抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

RAC1 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 22

RAC1 Antibody (PA1-091) in ICC/IF

Immunofluorescence analysis of RAC1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with RAC1 Polyclonal Antibody (Product # PA1-091X) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790)... View More

RAC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RAC1 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

RAC1 Antibody — Figure 1 Rac1 is overexpressed in breast cancer cells. (a) Upper panel: normal mammary epithelial cells (76N) and breast cancer cells of subtype luminal A (MCF-7 and T47D), and luminal B (BT-474 and ZR-75-1), triple-negative (MDA-MB-231 and MDA-MB-468) and HER2 positive (SkBr3, HCC1954, 21MT-1) were analyzed for Rac1 protein expression by Western blot analysis. As a protein loading control, the level of GAPDH in cell lysates was assessed. Lower panel: immunoblot densities of Rac1 and GAPDH were quantified using ImageJ analytical program (NIH) and relative Rac1 expression versus GAPDH determined. (b) Representative IHC analysis shows a distinct increase in immunostaining of Rac1 in malignant breast tumor tissues compared to normal breast tissues. (c) Box plot shows composite score of Rac1 expression in normal breast/benign tumor tissues (NT) and cancerous breast tissue (Cancer), analyzed by IHC. {TM}

Immunogen image

Immunogen Image RAC1 Polyclonal Antibody

产品信息

PA1-091

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

查看18篇已发表文章 18篇已发表文章

免疫组化 (IHC)

查看3篇已发表文章 3篇已发表文章

免疫组化（石蜡） (IHC (P))

1:20-1:50

免疫细胞化学 (ICC/IF)

1:20-1:100

查看4篇已发表文章 4篇已发表文章

免疫沉淀 (IP)

查看1篇已发表文章 1篇已发表文章

亲和纯化 (AP)

查看1篇已发表文章 1篇已发表文章

产品规格

已发表种属

C. elegans, Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant Full-length Human Protein

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2539856

靶标信息

The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。