Invitrogen

RAC1/RAC2/RAC3 Polyclonal Antibody

查看其他5个RAC1/RAC2/RAC3抗体

问题与答案

问题与答案

抗体检测数据 (7)
高级验证 (1)

RAC1/RAC2/RAC3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RAC1/RAC2/RAC3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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RAC1/RAC2/RAC3 Antibody (PA5-61600) in IHC (P)

Immunohistochemical analysis of RAC1/RAC2/RAC3 in human colon using RAC1/RAC2/RAC3 Polyclonal Antibody (Product # PA5-61600) shows weak to moderate membranous/ cytoplasmic positivity in glandular cells.

RAC1/RAC2/RAC3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RAC1/RAC2/RAC3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RAC1/RAC2/RAC3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RAC1/RAC2/RAC3 Antibody in Immunohistochemistry (IHC)

RAC1/RAC2/RAC3 Antibody in Western Blot (WB)

RAC1/RAC2/RAC3 Antibody in Western Blot (WB)

RAC1/RAC2/RAC3 Antibody in Western Blot (WB)

RAC1/RAC2/RAC3 Antibody

Product Image Gallery

RAC1/RAC2/RAC3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of RAC1/RAC2/RAC3 in human colon using RAC1/RAC2/RAC3 Polyclonal Antibody (Product # PA5-61600) shows weak to moderate membranous/ cytoplasmic positivity in glandular cells.

RAC1/RAC2/RAC3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of RAC1/RAC2/RAC3 in human pancreas using RAC1/RAC2/RAC3 Polyclonal Antibody (Product # PA5-61600) shows no positivity in exocrine glandular cells as expected.

RAC1/RAC2/RAC3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of RAC1/RAC2/RAC3 in human prostate using RAC1/RAC2/RAC3 Polyclonal Antibody (Product # PA5-61600) shows strong membranous positivity in basal cells.

RAC1/RAC2/RAC3 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of RAC1/RAC2/RAC3 in human esophagus using a RAC1/RAC2/RAC3 Polyclonal Antibody (Product # PA5-61600) shows strong cytoplasmic positivity in squamous epithelial cells.

RAC1/RAC2/RAC3 Antibody in Western Blot (WB) — Western blot was performed using Anti-RAC1/RAC2/RAC3 Polyclonal Antibody (Product # PA5-61600) and 21 kDa band corresponding to RAC1/RAC2/RAC3 was observed along with some higher glycosylated forms were also seen across samples tested. Whole cell extracts (30 µg lysate) of A-431 (Lane1), Jurkat (Lane2), U-87 MG (Lane 3), HEK-293 (Lane 4), HL-60 (Lane 5), Mouse Brain, (Lane 6), Mouse Thymus (Lane 7) and Mouse Spleen (Lane 8) were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris gel (Product # NP0342BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

RAC1/RAC2/RAC3 Antibody in Western Blot (WB) — Knockdown of RAC1/RAC2/RAC3 was achieved by transfecting HEK-293 cells with Rac1/Rac2/Rac3 specific siRNAs (Silencer® select Product # s11713 - RAC1, Product # s11716 & Product # s11714 - RAC2, Product # s11717 & Product # s224513 - RAC3). Western blot analysis (Fig. a) was performed using whole cell extracts from the RAC1/RAC2/RAC3 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed by RAC1/RAC2/RAC3 Polyclonal Antibody (Product # PA5-61600, 1:500 dilution) and Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4,000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific toRac1/Rac2/Rac3.

RAC1/RAC2/RAC3 Antibody in Western Blot (WB) — Western blot analysis of RAC1/RAC2/RAC3 in RT-4 cells. Samples were incubated with RAC1/RAC2/RAC3 polyclonal antibody (Product # PA5-61600).

RAC1/RAC2/RAC3 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HEK-293 cells were transfected with RAC1/RAC2/RAC3 siRNA and reduction of signal was observed in Western Blot using RAC1/RAC2/RAC3 Polyclonal Antibody (Product # PA5-61600). {KD}

产品信息

PA5-61600

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.04-0.4 µg/mL

-

免疫组化（石蜡） (IHC (P))

1:50-1:200

-

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein corresponding to Human RAC1/RAC2/RAC3

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.2, with 40% glycerol

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Immunogen sequence: TKLDLRDDKD TIERLRDKKL APITYPQGLA MAREI

Highest antigen sequence identity to the following orthologs: Mouse - 100%, Rat - 100%.

靶标信息

The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Cell migration-inducing gene 5 protein; GX; p21-Rac1; p21-Rac2; p21-Rac3; Ras-like protein TC25; Ras-related C3 botulinum toxin substrate 1; Ras-related C3 botulinum toxin substrate 2; Ras-related C3 botulinum toxin substrate 3; Ras-related C3 botulinum toxin substrate 3 (rho family, small GTP-binding protein Rac2); rho family, small GTP binding protein Rac3; Small G protein

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基因别名: EN-7; Gx; HSPC022; MIG5; p21-Rac1; p21-Rac2; Rac-1; RAC1; RAC2; RAC3; TC-25; TC25

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UniProt ID: (Human) P63000, (Human) P15153, (Human) P60763

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Entrez Gene ID: (Human) 5879, (Human) 5880, (Human) 5881

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GTPase activity protein binding GTP binding Rab GTPase binding enzyme binding protein kinase binding GTP-dependent protein binding thioesterase binding histone deacetylase binding Rho GDP-dissociation inhibitor binding protein kinase regulator activity calcium-dependent protein binding small GTPase

positive regulation of protein phosphorylation auditory receptor cell morphogenesis mast cell chemotaxis epithelial cell morphogenesis movement of cell or subcellular component inflammatory response hyperosmotic response cell adhesion cell-matrix adhesion G-protein coupled receptor signaling pathway small GTPase mediated signal transduction axon guidance blood coagulation cell proliferation regulation of cell size response to wounding anatomical structure morphogenesis regulation of hydrogen peroxide metabolic process positive regulation of lamellipodium assembly regulation of fibroblast migration positive regulation of cell-substrate adhesion regulation of neuron maturation cerebral cortex radially oriented cell migration embryonic olfactory bulb interneuron precursor migration cerebral cortex GABAergic interneuron development lamellipodium assembly actin cytoskeleton organization actin filament polymerization platelet activation regulation of cell migration positive regulation of actin filament polymerization T cell costimulation ruffle organization negative regulation of interleukin-23 production substrate adhesion-dependent cell spreading positive regulation of Rho protein signal transduction intracellular signal transduction Fc-epsilon receptor signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis positive regulation of apoptotic process positive regulation of phosphatidylinositol 3-kinase activity engulfment of apoptotic cell cell-cell junction organization bone resorption positive regulation of DNA replication vascular endothelial growth factor receptor signaling pathway ephrin receptor signaling pathway negative regulation of receptor-mediated endocytosis anatomical structure arrangement dendrite morphogenesis cell motility homeostasis of number of cells within a tissue regulation of defense response to virus by virus regulation of small GTPase mediated signal transduction positive regulation of stress fiber assembly localization within membrane positive regulation of focal adhesion assembly synaptic transmission, GABAergic Wnt signaling pathway, planar cell polarity pathway regulation of respiratory burst cellular response to mechanical stimulus semaphorin-plexin signaling pathway dopaminergic neuron differentiation protein localization to plasma membrane positive regulation of neutrophil chemotaxis cochlea morphogenesis ruffle assembly positive regulation of substrate adhesion-dependent cell spreading chemotaxis actin filament organization signal transduction positive regulation of cell proliferation regulation of cell-substrate adhesion cell projection assembly regulation of T cell proliferation regulation of mast cell degranulation regulation of protein kinase activity regulation of mast cell chemotaxis lymphocyte aggregation regulation of neutrophil migration positive regulation of protein targeting to mitochondrion neuron projection development positive regulation of cell adhesion mediated by integrin neuromuscular process controlling balance