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Invitrogen
RAD21 Monoclonal Antibody (CM110-2C10)
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RAD21 Antibody (MA1-16585) in WB
Western blot was performed using RAD21 Monoclonal Antibody (CM110-2C10) (Product # MA1-16585) and a band at ~90 kDa corresponding to RAD21 was decreased upon etoposide treatment in HeLa. Modified whole cell extracts (1% SDS) (30 µg lysate) of HeLa (Lane 1), HeLa treated with 50µM Etoposide for 48 hrs (Lane 2) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The b... View More
产品信息
MA1-16585
产品规格
种属反应
Human,
Mouse
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
CM110-2C10
抗原
An N-terminal fusion protein of the C-terminal fragment of Scc1 subunit human cohesin (amino acids 544-631 of hRad21).
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
PBS
内含物
0.1% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
In Western blot, two bands are seen: the full-length Rad21 is seen around 130 kDa, while the cleaved Rad21 is seen around 70 kDa.
Suggested positive control: antigen standard for RAD21 (transient overexpression lysate), Hela whole cell extract.
靶标信息
This gene encodes a nuclear protein that regulates transcriptional elongation and pre-mRNA splicing. The encoded protein interacts with the hyperphosphorylated C-terminal domain of RNA polymerase II via multiple FF domains, and with the pre-mRNA splicing factor SF1 via a WW domain. Alternative splicing results in multiple transcripts variants encoding different isoforms.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Double-strand-break repair protein rad21 homolog; FLJ25655; FLJ40596; h NXP-1; hHR21; KIAA0078RAD21; kleisin; mHR21; Nuclear matrix protein 1; NXP-1; Pokeweed agglutinin-binding protein 29; protein involved in DNA double-strand break repair; PW29; RAD21 homolog; SCC1 homolog; sister chromatid cohesion 1
基因别名: CDLS4; hHR21; HR21; HRAD21; KIAA0078; MCD1; mKIAA0078; NXP1; RAD21; SCC1
UniProt ID: (Human) O60216, (Mouse) Q61550
Entrez Gene ID: (Human) 5885, (Mouse) 19357
double-strand break repair
DNA recombination
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
apoptotic process
sister chromatid cohesion
mitotic nuclear division
reciprocal meiotic recombination
protein sumoylation
positive regulation of transcription from RNA polymerase II promoter
cell division
protein localization to chromatin
DNA repair
cellular response to DNA damage stimulus
cell cycle
chromosome segregation
