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Identifyn
RAD50 Polyclonal Antibody, Alexa Fluor™ 647, Identifyn™
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图: 1 / 8
RAD50 Antibody (DC-000061) in ICC/IF
Identifyn Detailed protocols can be found below under Documents & Downloads. Confocal Microscopy: HeLa cells were grown on acid-etched 1.5H cover glass to ~50% confluency, then fixed in -20°C methanol and blocked with Thermo Fisher Fish Serum Blocker (Product # 37527X3). The primary antibody, Rabbit anti-Human RAD50 Polyclonal - Identifyn SRM Alexa Fluor 647, IgG, was incubated for 1 hour at room temperature at a 1:250 dilution. Cells were washed 3 times in PBS. The second primary antibody, Nuclear Pore Complex Monoclonal Antibody (39C7), ... View More
产品信息
DC-000061
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Peptide Sequences between Residues 1286 - 1312, referencing Uniprot Q92878 and Gene ID 101111.
偶联物
激发/发射光谱
650/671 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
规格
50 µg
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 10% glycerol
内含物
0.02% sodium azide
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Identifyn™ Primary and Secondary antibodies are extensively validated in Immunofluorescence assays by traditional as well as high resolution acquisition like Widefield SIM, Confocal, Airyscan and STORM Microscopy.
Recommended dilution for traditional microscopy:
Widefield - 1:250-1:1,000
Widefield SIM - 1:250-1:1,000
Confocal - 1:250-1:1,000
Recommended dilution for Super Resolution microscopy:
SIM (Structured Illumination Microscopy) 1:100-1:1,000
Single Molecule STORM (Stochastic Optical Reconstruction Microscopy) - 1:100-1:1,000
Detailed protocols can be found below under Documents & Downloads.
靶标信息
Rad50-13B3 recognizes human Rad50, a 153a protein involved in DNA double strand break repair. Rad50 is associated with Mre11 and p95 (Nibrin) to form a multiprotein complex involved in the double strand break repair process. Upon DNA damage, Rad50 and Mre11 redistribute and are co-localized within the nucleus of damaged cells.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
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生物信息学
蛋白别名: DNA repair protein RAD50; hRAD50; hsRAD50; RAD50 2; RAD50 homolog, double strand break repair protein; RAD50 PEN; RAD50-2; RAD50-3; unnamed protein product
基因别名: hRad50; NBSLD; RAD50; RAD502
UniProt ID: (Human) Q92878
Entrez Gene ID: (Human) 10111
single-stranded DNA endodeoxyribonuclease activity
nucleotide binding
DNA binding
DNA helicase activity
double-stranded telomeric DNA binding
protein binding
ATP binding
3'-5' exonuclease activity
hydrolase activity
ATPase activity
protein binding, bridging
identical protein binding
single-stranded telomeric DNA binding
protein serine/threonine kinase activator activity
metal ion binding
G-quadruplex DNA binding
regulation of mitotic recombination
telomere maintenance via recombination
telomere maintenance
double-strand break repair via homologous recombination
DNA double-strand break processing
DNA repair
double-strand break repair
DNA recombination
cellular response to DNA damage stimulus
telomere maintenance via telomerase
reciprocal meiotic recombination
telomeric 3' overhang formation
positive regulation of telomere maintenance
reciprocal DNA recombination
mitotic G2/M transition checkpoint
chromosome organization
meiotic cell cycle
R-loop processing
chromosome organization involved in meiotic cell cycle
DNA strand resection involved in replication fork processing
negative regulation of telomere capping
positive regulation of double-strand break repair
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