Invitrogen

RAN Polyclonal Antibody

Name: RAN Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-79913

高级验证

查看其他19个RAN抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (15)
高级验证 (1)

RAN Antibody in Immunocytochemistry (ICC/IF)

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RAN Antibody (PA5-79913) in ICC/IF

Immunocytochemistry analysis of Ran using anti-Ran antibody (Product # PA5-79913) . Ran was detected in a section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-Ran antibody (Product # PA5-79913) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize... View More

RAN Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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RAN Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with RAN siRNA and decrease in signal intensity was observed in western blot application using Anti-RAN Polyclonal Antibody (Product # PA5-79913). {KD}

Immunogen image

Immunogen Image RAN Polyclonal Antibody

产品信息

PA5-79913

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

免疫组化（石蜡） (IHC (P))

0.5-1 µg/mL

免疫细胞化学 (ICC/IF)

2 µg/mL

流式细胞分析 (Flow)

1-3 µg/1x10^6 cells

免疫沉淀 (IP)

0.5-2 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E. coli-derived human Ran recombinant protein (Position: A2-L216).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Antigen affinity chromatography

保存液

4mg trehalose, 0.9mg NaCl, 0.2mg Na2PO4

内含物

0.01mg sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2747028

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: human PC-3 whole cell, human RT4 whole cell, human A549 whole cell, human CACO-2 whole cell, rat thymus tissue, rat PC-12 whole cell, mouse thymus tissue, mouse A20 whole cell. IHC: mouse small intestine tissue, rat small intestine tissue, human mammary cancer tissue, human intestinal cancer tissue, human lung cancer tissue. ICC/IF: U20S cell IP: HepG2 whole cell. Flow: A431 cell, U937 cell.

靶标信息

Ran (ras-related nuclear protein) is a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The Ran protein is also involved in control of DNA synthesis and cell cycle progression. Nuclear localization of Ran requires the presence of regulator of chromosome condensation 1 (RCC1).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Androgen receptor-associated protein 24; ARA25; GTP-binding nuclear protein Ran; GTPase Ran; guanosine triphosphatase Ran; member RAS oncogene family; OK/SW-cl.81; OTTHUMP00000166078; OTTHUMP00000166102; RanGTPase; Ras-like protein TC4; Ras-related nuclear protein

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基因别名: ARA24; Gsp1; OK/SW-cl.81; RAN; Rasl2-8; TC4

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UniProt ID: (Human) Q9CWI7, (Rat) P62828, (Mouse) P62827

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Entrez Gene ID: (Human) 5901, (Rat) 84509, (Mouse) 19384

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