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Invitrogen
RAP1A Polyclonal Antibody
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图: 1 / 3
RAP1A Antibody (PA5-106858) in ICC/IF
Immunocytochemistry analysis of RAP1A in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with RAP1A polyclonal antibody (Product # PA5-106858) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-106858
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human RAP1A(Accession P62834), corresponding to amino acid residues I152-L184.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
1 mg/mL
规格
100 µg
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Antibody detects endogenous levels of total RAP1A.
靶标信息
The product of this gene belongs to the family of RAS-related proteins. These proteins share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between RAP proteins and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. The product of this gene counteracts the mitogenic function of RAS because it can interact with RAS GAPs and RAF in a competitive manner. Two transcript variants encoding the same protein have been identified for this gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: C21KG; G-22K; GTP-binding protein smg p21A; GTP-binding protein SMG-P21A; RAS-related protein 1a; Ras-related protein Krev-1; Ras-related protein Rap-1A
基因别名: AI848598; G-22K; Krev-1; KREV1; rap 1A; RAP-1A; Rap1; RAP1A
UniProt ID: (Mouse) P62835, (Rat) P62836
Entrez Gene ID: (Mouse) 109905, (Rat) 295347
signal transduction
small GTPase mediated signal transduction
nervous system development
positive regulation of neuron projection development
protein transport
Rap protein signal transduction
nerve growth factor signaling pathway
positive regulation of GTPase activity
positive regulation of protein kinase activity
establishment of endothelial barrier
positive regulation of ERK1 and ERK2 cascade
cellular response to cAMP
regulation of cell junction assembly
cellular response to nerve growth factor stimulus
negative regulation of synaptic vesicle exocytosis
positive regulation of vasculogenesis
metabolic process
microvillus assembly
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