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Invitrogen
RAP1A Polyclonal Antibody
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FIGURE: 1 / 5
RAP1A Antibody (PA5-79914) in ICC/IF
Immunofluorescence analysis of RAP1A was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with RAP1A Polyclonal Antibody (Product PA5-79914) at 1 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then with Goat anti-Rabbit IgG (Heavy Chain), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 (Product # A27034, 1:2000 dilut... View More
Product Details
PA5-79914
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human RAP1A (156-181aa EIFYDLVRQINRKTPVEKKKPKKKSC).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: Rat Brain Tissue, Rat Liver Tissue, Rat Kidney Tissue, MCF-7 whole cell, SW620 whole cell, rat heart tissue, rat lung tissue, rat stomach tissue, rat kidney tissuemouse heart tissue, mouse lung tissue, mouse stomach tissue, mouse kidney tissue.
Target Information
The product of this gene belongs to the family of RAS-related proteins. These proteins share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between RAP proteins and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. The product of this gene counteracts the mitogenic function of RAS because it can interact with RAS GAPs and RAF in a competitive manner. Two transcript variants encoding the same protein have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C21KG; G-22K; GTP-binding protein smg p21A; GTP-binding protein SMG-P21A; RAS-related protein 1a; Ras-related protein Krev-1; Ras-related protein Rap-1A; small GTP binding protein; unnamed protein product
Gene Aliases: AI848598; C21KG; G-22K; KREV-1; KREV1; rap 1A; RAP-1A; RAP1; RAP1A; SMGP21
UniProt ID: (Human) P62834, (Mouse) P62835, (Rat) P62836
Entrez Gene ID: (Human) 5906, (Mouse) 109905, (Rat) 295347
nucleotide binding
GTPase activity
obsolete small monomeric GTPase activity
guanyl-nucleotide exchange factor activity
protein binding
GTP binding
hydrolase activity
GDP binding
small GTPase binding
macromolecular complex binding
protein transporter activity
Ras GTPase binding
Rap guanyl-nucleotide exchange factor activity
protein complex binding
small GTPase
signal transduction
small GTPase mediated signal transduction
nervous system development
response to carbohydrate
positive regulation of neuron projection development
synaptic vesicle exocytosis
Rap protein signal transduction
negative regulation of collagen biosynthetic process
nerve growth factor signaling pathway
positive regulation of GTPase activity
positive regulation of protein kinase activity
positive regulation of glucose import
positive regulation of phagocytosis
positive regulation of Fc receptor mediated stimulatory signaling pathway
establishment of endothelial barrier
positive regulation of ERK1 and ERK2 cascade
cellular response to cAMP
protein localization to plasma membrane
response to antineoplastic agent
liver regeneration
regulation of neurotransmitter receptor localization to postsynaptic specialization membrane
regulation of cell junction assembly
cellular response to forskolin
cellular response to nerve growth factor stimulus
negative regulation of synaptic vesicle exocytosis
positive regulation of vasculogenesis
protein transport
metabolic process
microvillus assembly
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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