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Invitrogen
RBCK1 Polyclonal Antibody
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图: 1 / 2
RBCK1 Antibody (PA5-115828) in ICC/IF
Immunocytochemistry analysis of RBCK1 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115828). Secondary staining was applied with mouse anti-beta tubulin (1 hr at 37°, AlexaFluor 594 conjugated goat anti-rabbit IgG (Red), AlexaFluor 488 conjugated goat anti-mouse IgG (Green) and DAPI.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-115828
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human RBCK1(Accession Q9BYM8), corresponding to amino acid residues K477-H510.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
1 mg/mL
规格
100 µg
纯化类型
Affinity chromatography
保存液
PBS with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Antibody detects endogenous levels of total RBCK1.
靶标信息
RBCK1 encodes a protein that is similar to mouse UIP28/UbcM4 interacting protein. Alternative splicing has been observed at this locus, resulting in distinct isoforms.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: HBV-associated factor 4; Heme-oxidized IRP2 ubiquitin ligase 1; Heme-oxidized IRP2 ubiquitin ligase 1 homolog; Hepatitis B virus X-associated protein 4; HOIL-1; novel zinc finger, C3HC4 type (RING finger) protein; Protein kinase C-binding protein beta-15; protein kinase C-binding protein Beta15; RanBP-type and C3HC4-type zinc finger-containing protein 1; RBCC protein interacting with PKC; RBCC protein interacting with PKC 1; RING finger protein 54; RING-type E3 ubiquitin transferase HOIL-1; UbcM4-interacting protein 28; ubiquitin conjugating enzyme 7 interacting protein 3; Ubiquitin-conjugating enzyme 7-interacting protein 3
基因别名: AL033326; C20orf18; HOIL-1; HOIL-1L; Pkcbpb15; Rbck; RBCK1; RNF54; UBCE7IP3; UIP28; XAP3; XAP4
UniProt ID: (Rat) Q62921, (Mouse) Q9WUB0
Entrez Gene ID: (Rat) 60383, (Mouse) 24105
double-stranded DNA binding
ubiquitin-protein transferase activity
protein kinase C binding
protein binding
zinc ion binding
ligase activity
ubiquitin binding
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
metal ion binding
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
ubiquitin-protein ligase
protein modifying enzyme
protein polyubiquitination
negative regulation of NF-kappaB transcription factor activity
positive regulation of NF-kappaB import into nucleus
positive regulation of apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
proteasome-mediated ubiquitin-dependent protein catabolic process
positive regulation of transcription from RNA polymerase II promoter
T cell receptor signaling pathway
positive regulation of NF-kappaB transcription factor activity
negative regulation of necroptotic process
protein linear polyubiquitination
positive regulation of extrinsic apoptotic signaling pathway
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