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RBM3 Antibody (MA5-50678) in ICC/IF
Immunocytochemistry analysis of RBM3 in HeLa cells. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 , permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were incubated with RBM3 recombinant monoclonal antibody (Product # MA5-50678) with a dilution of 1:50 in 2% negative goat serum overnight at 4 . Followed by secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at a dilution of 1:1,000. Nuclear DNA was labelled in blue wi... View More
产品信息
MA5-50678
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
JE63-84
抗原
Recombinant protein within human RBM3 aa 58-157/157.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
TBS, pH 7.4, with 0.05% BSA, 40% glycerol
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_3094132
产品详细信息
Positive control: Hela, human esophagus tissue, mouse thyroid tissue, mouse trachea tissue, rat trachea tissue, rat esophagus tissue, THP-1.
Predicted band size: 17 kDa
Subcellular Location: Nucleus, cytoplasm, dendrite.
靶标信息
RBM3 is a member of the glycine-rich RNA-binding protein family and a protein with one RNA recognition motif (RRM) domain. Expression of this protein is induced by cold shock and low oxygen tension.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: OTTHUMP00000025800; OTTHUMP00000025802; putative RNA-binding protein 3; RNA binding motif protein 3; RNA-binding motif protein 3; RNA-binding protein 3; RNPL
基因别名: 2600016C11Rik; IS1-RNPL; RBM3; RNPL
UniProt ID: (Human) P98179, (Mouse) O89086, (Rat) Q925G0
Entrez Gene ID: (Human) 5935, (Mouse) 19652, (Rat) 114488
