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Agrisera
RBOHD/Respiratory burst oxidase homolog protein D Polyclonal Antibody
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RBOHD/Respiratory burst oxidase homolog protein D Antibody (AS15-2962) in WB
Total proteins were isolated from 10 or 12 seedlings of 7-day-old Arabidopsis thaliana seedlings of Col-0 (wild-type) and rbohd null mutant (Nühse et al., 2007). 16 µL of total proteins were denatured at 65°C for 5 min, separated on an 8% SDS-PAGE. and transferred for 70 min at 55 V using a tank transfer system to nitrocellulose membrane. Blots were blocked with 1 x PBS+0.1% Tween 20 + 5% milk (PBS-T) or TB... View More
产品信息
AS15-2962
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated peptide chosen from Arabidopsis thaliana RBOHD sequence.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana, Solanum sp.
靶标信息
RBOHD encodes a plant NADPH oxidase (respiratory burst oxidase homolog D) that localizes predominantly to the plasma membrane, where it catalyzes extracellular/apoplastic reactive oxygen species (ROS) production as part of rapid stress and immune signaling. The protein is a large multi-pass membrane flavocytochrome with a long cytosolic N-terminus containing two EF-hand Ca2+-binding motifs and regulatory regions targeted by phosphorylation, coupled to a C-terminal dehydrogenase module bearing conserved FAD- and NADPH-binding sites; between these cytosolic regions, multiple (classically six) transmembrane helices form the membrane anchor and support heme-binding elements characteristic of NOX-family enzymes that transfer electrons from cytosolic NADPH across the membrane to molecular oxygen to generate superoxide that can dismutate to hydrogen peroxide. Biologically, RBOHD is a major source of stimulus-induced ROS during diverse responses, including pattern-triggered immunity and abiotic stress, contributing to localized defense outputs (e.g., oxidative burst-associated cell wall and signaling changes) and enabling rapid cell-to-cell and systemic signal propagation in response to wounding, temperature shifts, high light, salinity, and related cues.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AT5G47910; AtRboh04; ATRBOHD; MCA23.25; MCA23_25; RESPIRATORY BURST OXIDASE
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