Invitrogen

RBPJ Recombinant Rabbit Monoclonal Antibody (9B3L6)

查看其他12个RBPJ抗体

问题与答案

问题与答案

抗体检测数据 (3)

RBPJ Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RBPJ Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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RBPJ Antibody (MA5-35438) in IHC (P)

Immunohistochemistry analysis of RBPJ in paraffin-embedded human lung squamous carcinoma tissue. Samples were incubated with RBPJ Monoclonal antibody (Product # MA5-35438) using a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

RBPJ Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RBPJ Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RBPJ Antibody in Western Blot (WB)

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RBPJ Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of RBPJ in paraffin-embedded human lung squamous carcinoma tissue. Samples were incubated with RBPJ Monoclonal antibody (Product # MA5-35438) using a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

RBPJ Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of RBPJ in paraffin-embedded human colon carcinoma. Samples were incubated with RBPJ Monoclonal antibody (Product # MA5-35438) using a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

RBPJ Antibody in Western Blot (WB) — Western blot analysis of RBPJ in extracts of various cell lines. Samples were incubated with RBPJ Monoclonal antibody (Product # MA5-35438) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunogen image

RBPJ Recombinant Rabbit Monoclonal Antibody (9B3L6)

Immunogen Image RBPJ Recombinant Rabbit Monoclonal Antibody (9B3L6)

产品信息

MA5-35438

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:6,000

-

免疫组化（石蜡） (IHC (P))

1:100-1:500

-

酶联免疫吸附实验 (ELISA)

1 µg/mL

-

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

9B3L6

抗原

A synthetic peptide corresponding to a sequence within amino acids 100-200 of human RBPJK (Q06330)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.20 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

内含物

0.02% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Immunogen sequence: CAFIGIGNSD QEMQQLNLEG KNYCTAKTLY ISDSDKRKHF MLSVKMFYGN SDDIGVFLSK RIKVISKPSK KKQSLKNADL CIASGTKVAL FNRLRSQTVS T

靶标信息

CSL plays a role in the regulatory network through which muscle cells coordinate their structural and functional states during growth, adaptation, and repair. The protein is abundantly expressed in heart and skeletal muscle and expression is increased by passive stretch. Mutations in this gene can lead to X-linked deafness.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: CBF-1; H-2K binding factor-2; immunoglobulin kappa J region recombination signal binding protein 1; J kappa-recombination signal-binding protein; RBP-J; RBP-J kappa; RBP-JK; rbp-jkappa; Recombining binding protein suppressor of hairless; Renal carcinoma antigen NY-REN-30; suppressor of hairless homolog

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基因别名: AI843960; AOS3; CBF1; csl; IGKJRB; IGKJRB1; Igkrsbp; KBF2; RBP-J; RBP-J kappa; RBPJ; RBPJK; RBPSUH; SUH

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UniProt ID: (Human) Q06330, (Mouse) P31266

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Entrez Gene ID: (Human) 3516, (Mouse) 19664

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recombinase activity RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II repressing transcription factor binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding protein binding transcription factor binding sequence-specific DNA binding protein N-terminus binding transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding protein complex binding DNA-binding transcription factor

negative regulation of transcription from RNA polymerase II promoter angiogenesis somitogenesis epithelial to mesenchymal transition blood vessel remodeling inflammatory response to antigenic stimulus secondary heart field specification outflow tract morphogenesis endocardium morphogenesis epithelial to mesenchymal transition involved in endocardial cushion formation cardiac left ventricle morphogenesis ventricular trabecula myocardium morphogenesis regulation of transcription from RNA polymerase II promoter involved in myocardial precursor cell differentiation DNA recombination transcription initiation from RNA polymerase II promoter humoral immune response Notch signaling pathway positive regulation of transcription of Notch receptor target negative regulation of cell proliferation auditory receptor cell fate commitment epidermal cell fate specification pituitary gland development B cell differentiation keratinocyte differentiation negative regulation of ossification positive regulation of BMP signaling pathway somatic stem cell population maintenance dorsal aorta morphogenesis atrioventricular canal development defense response to bacterium myeloid dendritic cell differentiation negative regulation of cell differentiation negative regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter regulation of timing of cell differentiation sebaceous gland development hair follicle maturation positive regulation of cardiac muscle cell proliferation Clara cell differentiation labyrinthine layer blood vessel development arterial endothelial cell fate commitment Notch signaling involved in heart development positive regulation of transcription from RNA polymerase II promoter in response to hypoxia blood vessel lumenization interleukin-4 secretion blood vessel endothelial cell fate specification positive regulation of ERBB signaling pathway positive regulation of ephrin receptor signaling pathway positive regulation of canonical Wnt signaling pathway involved in cardiac muscle cell fate commitment positive regulation of cell proliferation involved in heart morphogenesis heart looping endocardium development transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter heart development positive regulation of cell proliferation regulation of gene expression positive regulation of gene expression negative regulation of neuron projection development negative regulation of smooth muscle cell migration hemopoiesis neuron differentiation negative regulation of smooth muscle cell apoptotic process positive regulation of Notch signaling pathway involved in heart induction cell fate commitment positive regulation of transcription, DNA-templated positive regulation of smooth muscle cell proliferation artery morphogenesis Notch signaling pathway involved in arterial endothelial cell fate commitment regulation of cell adhesion involved in heart morphogenesis determination of heart left/right asymmetry