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Agrisera
RDR6/RNA-dependent RNA polymerase 6 Polyclonal Antibody
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RDR6/RNA-dependent RNA polymerase 6 Antibody (AS15-3098) in WB
50 µg of total protein from Arabidopsis thaliana whole vegetative rosette wild type Col-0(a)rdr 6-12 mutant (b)extracted with extraction buffer (50 m M Tris p H 7.5; 150 m M Na Cl; 1 m M EDTA; 10% v/v Glycerin; 1 m M DTT, 1 x Complete Protease Inhibitor Cocktail, Roche) and denatured with LaemmLi buffer at 95°C/5 min., were separated on 7.5% SDS-PAGE and blotted 1.5 h to PVDF using tank transfer. Blots were... View More
产品信息
AS15-3098
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated peptide derived from Arabidopsis thaliana RDR6 sequence.
偶联物
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 200 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
RDR6 encodes a cytoplasmic RNA-dependent RNA polymerase that functions in plant RNA silencing, acting downstream of small-RNA targeting to convert single-stranded RNA templates into double-stranded RNA that is subsequently processed into secondary siRNAs to reinforce and spread post-transcriptional gene silencing, including trans-acting siRNA biogenesis and antiviral defense. The encoded protein is a eukaryotic-type RdRP with a conserved catalytic core characteristic of RNA polymerases, including the essential Asp residue required for polymerase activity, and it also exhibits terminal nucleotidyltransferase activity and primer-independent as well as primer-dependent initiation on RNA templates in vitro. Structurally, RDR6 is a large, multi-domain protein containing an N-terminal RNA recognition motif-like region and a C-terminal RdRP domain that supports dsRNA synthesis needed for amplification (transitivity) of silencing signals and robust maintenance of silencing across tissues.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AtRDR6; SDE1; SGS2; SILENCING DEFECTIVE 1; SUPPRESSOR OF GENE SILENCING 2
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