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Invitrogen
RECQL5 Polyclonal Antibody
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FIGURE: 1 / 9
RECQL5 Antibody (PA5-115832) in ICC/IF
Immunocytochemistry analysis of RECQL5 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115832) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
Product Details
PA5-115832
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human RECQL5(Accession O94762), corresponding to amino acid residues E808-S858.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total RECQL5.
Target Information
RECQL5 gene ontology annotations related to this gene include cellular response to camptothecin; chromosome separation; DNA duplex unwinding; DNA recombination; DNA repair; DNA replication; double-strand break repair via homologous recombination; mitotic sister chromatid segregation; negative regulation of double-strand break repair via homologous recombination; negative regulation of mitotic cell cycle; negative regulation of transcription elongation from RNA polymerase II promoter; replication-born double-strand break repair via sister chromatid exchange; response to X-ray.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATP-dependent DNA helicase Q5; DNA 3'-5' helicase RecQ5; DNA helicase, RecQ-like type 5; FLJ90603; RecQ helicase-like 5; RecQ protein 5; RecQ protein-like 5; RecQ5; RECQL5; RECQL5beta; unnamed protein product
Gene Aliases: RECQ5; Recq5b; RECQL5; Recql5b
UniProt ID: (Human) O94762, (Rat) D4ACP5, (Mouse) Q8VID5
Entrez Gene ID: (Human) 9400, (Rat) 287834, (Mouse) 170472
nucleotide binding
RNA polymerase II core binding
nucleic acid binding
DNA binding
DNA helicase activity
helicase activity
protein binding
ATP binding
four-way junction helicase activity
hydrolase activity
isomerase activity
ATPase activity
identical protein binding
3'-5' DNA helicase activity
metal ion binding
catalytic activity, acting on DNA
ATP-dependent helicase activity
DNA helicase
mitotic cell cycle
double-strand break repair via homologous recombination
DNA metabolic process
DNA replication
DNA repair
DNA recombination
regulation of transcription, DNA-templated
cellular response to DNA damage stimulus
negative regulation of macromolecule metabolic process
negative regulation of transcription elongation from RNA polymerase II promoter
negative regulation of nucleobase-containing compound metabolic process
cell division
chromosome separation
cellular response to xenobiotic stimulus
cellular response to camptothecin
replication-born double-strand break repair via sister chromatid exchange
mitotic DNA-dependent DNA replication
negative regulation of double-strand break repair via homologous recombination
mitotic nuclear division
DNA duplex unwinding
cell cycle
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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