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Bethyl Laboratories
REDD1 Polyclonal Antibody
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FIGURE: 1 / 2
REDD1 Antibody (A302-169A) in ICC/IF
Detection of human REDD1 by immunocytochemistry. Sample: NBF-fixed asynchronous, Cobalt-treated HeLa cells. Antibody: Affinity purified rabbit anti-REDD1 (Product # A302-169A) (lot 1) used at a dilution of 1:500 (2 µg/mL). Detection: Red-fluorescent goat anti-rabbit IgG H&L cross-adsorbed Antibody DyLight®594 used at a dilution of 1:100.
Product Details
A302-169A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 1 and 50 of Human protein regulated in development and DNA damage response 1 (DNA-damage-inducible transcript 4).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Application Note: For ICC, formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
Based on 100% sequence identity, this antibody is predicted to react with Mouse, Rat, Bovine, Dog, Horse, Rabbit, Panda, Orangutan, Monkey, Gorilla and Chimpanzee
Target Information
DDIT4 gene ontology annotations related to this gene include cellular response to dexamethasone stimulus; intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator; negative regulation of glycolytic process; negative regulation of TOR signaling; protein-containing complex disassembly; reactive oxygen species metabolic process; response to hypoxia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA damage-inducible transcript 4 protein; DNA-damage-inducible transcript 4; HIF-1 responsive protein RTP801; Protein regulated in development and DNA damage response 1; REDD-1; RP11-442H21.1
Gene Aliases: DDIT4; Dig2; REDD-1; REDD1; RTP801
UniProt ID: (Human) Q9NX09
Entrez Gene ID: (Human) 54541
response to hypoxia
neuron migration
brain development
cell proliferation
negative regulation of peptidyl-threonine phosphorylation
neuron differentiation
negative regulation of TOR signaling
negative regulation of peptidyl-serine phosphorylation
intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
protein complex disassembly
negative regulation of glycolytic process
neurotrophin TRK receptor signaling pathway
defense response to virus
reactive oxygen species metabolic process
positive regulation of neuron death
negative regulation of intracellular signal transduction
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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