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Invitrogen
REST Recombinant Rabbit Monoclonal Antibody (JE32-25)
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图: 1 / 8
REST Antibody (MA5-44189) in ICC/IF
Immunofluorescence analysis of REST was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with REST Recombinant Rabbit Monoclonal Antibody (JE32-25) (Product # MA5-44189) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alex... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
MA5-44189
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
JE32-25
抗原
Recombinant protein within Human REST aa 850-1000.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
1 mg/mL
规格
100 µg
纯化类型
Protein A
保存液
TBS with 40% glycerol, 0.05% BSA
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Predicted band size: 122 kDa
Positive Control: HL-60 cell lysate, HUVEC cell lysate, human thyroid tissue, human seminoma tissue, SH-SY5Y.
靶标信息
Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: neural-restrictive silencer factor; neuron restrictive silencer factor; repressor binding to the X2 box; transcription factor
基因别名: DFNA27; GINGF5; HGF5; NRSF; WT6; XBR
UniProt ID: (Human) Q13127
Entrez Gene ID: (Human) 5978
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II core promoter sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
protein binding
identical protein binding
sequence-specific DNA binding
RNA polymerase II sequence-specific DNA binding transcription factor binding
negative regulation of transcription from RNA polymerase II promoter
regulation of alternative mRNA splicing, via spliceosome
response to hypoxia
response to ischemia
chromatin remodeling
regulation of transcription, DNA-templated
response to stress
positive regulation of gene expression
negative regulation of gene expression
negative regulation of aldosterone biosynthetic process
somatic stem cell population maintenance
positive regulation of programmed cell death
negative regulation by host of viral transcription
negative regulation of neuron differentiation
positive regulation of neuron differentiation
regulation of osteoblast differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
negative regulation of calcium ion-dependent exocytosis
negative regulation of insulin secretion
negative regulation of neurogenesis
neurological system process
neuromuscular process controlling balance
detection of mechanical stimulus involved in sensory perception of sound
auditory receptor cell stereocilium organization
cardiac muscle cell myoblast differentiation
cellular response to electrical stimulus
cellular response to glucocorticoid stimulus
neuronal stem cell population maintenance
modification of synaptic structure
positive regulation of stem cell population maintenance
negative regulation of pri-miRNA transcription from RNA polymerase II promoter
negative regulation of cortisol biosynthetic process
negative regulation of dense core granule biogenesis
negative regulation of mesenchymal stem cell differentiation
negative regulation of amniotic stem cell differentiation
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