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Agrisera
RFP/Red flourescent protein (mRFP, mCherry, tdTomato, mScarlet) Polyclonal Antibody
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RFP/Red flourescent protein (mRFP, mCherry, tdTomato, mScarlet) Antibody (AS24-5006) in IHC (P)
Type of material: Mouse intestinal FFPE tissue overexpressing m Scarlet tagged protein Fixation: formaldehyde Hydrophilization: n/a Cell wall digestion: n/a Membrane permeabilization: n/a Antigen retrieval: in citrate buffer Blocking buffer: Superblock plus (Thermo scientific 37,580) Washing buffer: PBS Primary antibody dilution and incubation time: 1:200/ 1 h incubation Secondary antibody: Vector labs, BA-... View More
产品信息
AS24-5006
产品规格
种属反应
Tag
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
Recombinant protein produced in E.coli corresponding to full length mRFP.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: RFP
靶标信息
RFP usually refers to red fluorescent protein used as a reporter and fusion tag in plant biology to visualize gene expression, protein localization, and dynamics in living cells. Derived from coral and engineered variants, RFP emits red fluorescence that penetrates tissues well and minimizes overlap with green reporters like GFP, enabling multicolor imaging. It can be targeted to specific compartments via signal peptides, linked to promoters for transcriptional readouts, or fused to proteins to track trafficking and interactions. RFP is generally monomeric and photostable in modern variants, but brightness, maturation speed, pH sensitivity, and photobleaching differ among types, so choice of variant and imaging settings are important. Controls for autofluorescence, expression level, and proper fusion design help ensure accurate interpretation in developmental and stress studies.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Mmt; Rfn; Rfp; Rfp1
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