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Agrisera
RGA/DELLA protein RGA Polyclonal Antibody
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RGA/DELLA protein RGA Antibody (AS11-1630) in WB
Arabidopsis thaliana seedlings were ground in liquid nitrogen (100 µL of 2.5 x LaemLi for 80-120 mg of homogenized material) and boiled in 2,5 x LaemmLi Buffer (WITH 60 Mm DTT final concentration) otherwise RGA protein will degrade. Plants were grown on 1/ MS for 15 days and were treated with 1 µM GA for 2 hours (GA+) or without hormone (GA-). Total protein extracts were denatured for 2 min. at 95°C and wer... View More
产品信息
AS11-1630
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated peptide chosen from RGA of Arabidopsis thaliana.
偶联物
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 100 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
RGA, also called repressor of ga1 3 like, is a DELLA protein that represses gibberellin signaling in plants. In the absence of gibberellin, RGA accumulates in the nucleus and binds transcription factors to inhibit growth processes such as stem elongation, seed germination, and flowering. When gibberellin levels rise, the receptor GID1 promotes interaction of RGA with the SCF E3 ligase containing SLY1 or GID2, leading to ubiquitination and proteasomal degradation of RGA and activation of GA responsive genes. RGA integrates cross talk with auxin, brassinosteroids, and light signaling, and its stability and activity are modulated by phosphorylation and other posttranslational modifications. Mutations affecting RGA alter plant stature, developmental timing, and stress responses by shifting the balance of growth repression and hormone sensitivity.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AT2G01570; F2I9.19; F2I9_19; Q9SLH3; REPRESSOR OF GA; REPRESSOR OF GA1-3 1; RGA; RGA1
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