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Invitrogen
RGS21 Polyclonal Antibody
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RGS21 Antibody (PA5-104094) in ICC/IF
Immunocytochemistry analysis of RGS21 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with RGS21 polyclonal antibody (Product # PA5-104094) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
产品信息
PA5-104094
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human RGS21(Accession Q2M5E4), corresponding to amino acid residues M1-E49.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Antibody detects endogenous levels of total RGS21.
靶标信息
Regulator of G-protein signaling (RGS) proteins contain an 120 amino acid conserved domain, termed the RGS domain, that acts as a GTPase-activating protein that acts to reduce the signal transmitted by the receptor-activated G-alpha subunit. RGS21 is a recently identified member of this family that has been reported to be selectively expressed in subpopulations of taste bud cells and co-expressed with bitter and sweet transduction components such as alpha-gusticin, phospholipase Cbeta2, T1R2/T1R3 sweet taste receptors and T2R bitter taste receptors. Other reports indicate that RGS21 is more widely expressed. Binding assays demonstrate that RGS21 binds alpha-gusticin in a conformation-dependent manner and may do the same with T1R receptors, suggesting that RGS21 may play a role in sweet and bitter taste transduction processes.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Regulator of G-protein signaling 21; regulator of G-protein signalling 21; RGS21
基因别名: EG624910; RGS21
UniProt ID: (Human) Q2M5E4
Entrez Gene ID: (Human) 431704, (Rat) 100361166, (Mouse) 624910
