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Agrisera
RH3/RNA helicase (chloroplastic) Polyclonal Antibody
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RH3/RNA helicase (chloroplastic) Antibody (AS13-2714) in WB
5 µg of total seedling leaf protein or from the indicated subcellular fractions from Zea mays were separated on 12% SDS-PAGE and blotted to nitrocellulose. Blots were blocked with 4% milk for 1 h at room temperature (RT) with agitation. The blot was incubated in the primary antibody at a dilution of 1:1,000 for 2 h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twic... View More
产品信息
AS13-2714
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
Recombinant RH3 from Zea mays, amino acids 531-691, conserved in Zea mays and Arabidopsis thaliana.
偶联物
形式
Lyophilized
保存液
whole serum
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana, Zea mays
靶标信息
RH3 is a plant DEAD box RNA helicase localized to chloroplasts that promotes ribosome biogenesis and RNA metabolism. It unwinds or remodels structured RNAs and ribonucleoprotein complexes to aid rRNA processing, assembly of chloroplast ribosomal subunits, and maturation of specific mRNAs. RH3 activity supports chloroplast translation, photosystem biogenesis, and overall photosynthetic capacity, with expression linked to chloroplast development and light conditions. Mutations in RH3 impair rRNA processing, reduce plastid protein synthesis, and lead to chlorosis, growth defects, and sensitivity to environmental stress due to compromised chloroplast function.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AtRH3; emb1138; embryo defective 1138
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