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Invitrogen
RHOT1 Polyclonal Antibody
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RHOT1 Antibody (PA5-143948) in ICC/IF
Immunocytochemistry analysis of RHOT1 in T-47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in RHOT1 Polyclonal antibody (Product # PA5-143948) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate ... View More
产品信息
PA5-143948
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived human MIRO1/RHOT1 recombinant protein (Position: Q63-A596).
偶联物
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 4mg trehalose
内含物
no preservative
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human HepG2 whole cell, human THP-1 whole cell, rat liver tissue, mouse heart tissue, mouse liver tissue. IHC: human thyroid papillary carcinoma tissue, human gall bladder adenosquamous carcinoma tissue, human laryngeal squamous cell carcinoma tissue, human lung cancer tissue. ICC/IF: T47D cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
靶标信息
Mitochondrial GTPase involved in mitochondrial trafficking. RHOT1 is probably involved in control of anterograde transport of mitochondria and their subcellular distribution.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: MIRO-1; mitochondrial Rho (MIRO) GTPase 1; Mitochondrial Rho GTPase 1; rac-GTP binding protein-like protein; Rac-GTP-binding protein-like protein; Ras homolog gene family member T1; ras homolog gene family, member T1
基因别名: 2210403N23Rik; AA415293; AF244542; AI834919; ARHT1; C430039G08Rik; MIRO-1; MIRO1; RHOT1
UniProt ID: (Human) Q8IXI2, (Mouse) Q8BG51
Entrez Gene ID: (Human) 55288, (Rat) 303351, (Mouse) 59040
