Invitrogen

RICTOR Monoclonal Antibody (J.644.6)

查看其他21个RICTOR抗体

问题与答案

问题与答案

问题与答案

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抗体检测数据 (3)
高级验证 (3)

RICTOR Antibody in Western Blot (WB)

RICTOR Antibody in Western Blot (WB)

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RICTOR Antibody (MA5-15006) in WB

Western blot was performed using Anti-RICTOR Polyclonal Antibody (Product # MA5-15006) and a 192 kDa band corresponding to RICTOR was observed across cell lines. Whole cell extracts (40 µg lysate) of SW480 (Lane 1), BeWo (Lane 2), HeLa (Lane 3), MOLT4 (Lane 4), HCT 116 serum starved overnight (Lane 5), HCT116 serum starved overnight and treated with EGF (20 ng/mL for 45 mins) (Lane 6), HCT116 serum starved overnight, treated with Curcumin 25 µM for 2 hrs and then treated with EGF (20 ng/mL for 45 mins) (Lane 7), A549 (Lane 8), A549 treated... View More

RICTOR Antibody in Western Blot (WB)

RICTOR Antibody in Western Blot (WB)

RICTOR Antibody in Western Blot (WB)

RICTOR Antibody

RICTOR Antibody

RICTOR Antibody

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

RICTOR Antibody in Western Blot (WB) — Western blot was performed using Anti-RICTOR Polyclonal Antibody (Product # MA5-15006) and a 192 kDa band corresponding to RICTOR was observed across cell lines. Whole cell extracts (40 µg lysate) of SW480 (Lane 1), BeWo (Lane 2), HeLa (Lane 3), MOLT4 (Lane 4), HCT 116 serum starved overnight (Lane 5), HCT116 serum starved overnight and treated with EGF (20 ng/mL for 45 mins) (Lane 6), HCT116 serum starved overnight, treated with Curcumin 25 µM for 2 hrs and then treated with EGF (20 ng/mL for 45 mins) (Lane 7), A549 (Lane 8), A549 treated with MG132 5 µM for 3 hrs (Lane 9) were electrophoresed using NuPAGE™ 3-8% Tris-Acetate Protein Gel (Product # EA0378BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (*1:1000) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:20000 dilution) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Atto Ultimate Sensitivity Substrate (Product # A38556).

RICTOR Antibody in Western Blot (WB) — Western Blot was performed using Anti-RICTOR Monoclonal Antibody (J.644.6) (Product # MA5-15006) and a 200 kDa band corresponding to Rapamycin-insensitive companion of mTOR was observed in A549 cells treated with MG132 (5 µM for 4hr). Freshly prepared Whole cell extracts (30 µg lysate) of A549 (Lane 1), A549 treated with MG132 (5 µM for 4hr) (Lane 2) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The Blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

RICTOR Antibody in Western Blot (WB) — Knockdown of RICTOR was achieved by transfecting HeLa with RICTOR specific siRNAs (Silencer® select Product # s48408, S226001). Western blot analysis (Fig. a) was performed using Whole cell extracts from the RICTOR knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with RICTOR Polyclonal Antibody (Product # MA5-15006, 1:1000) and Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:20000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to RICTOR.

RICTOR Antibody — Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western blot using RICTOR Monoclonal Antibody (J.644.6) (Product # MA5-15006), shows expression of RICTOR in A549 cells treated with MG132 (5 µM for 4hr). {TM}

RICTOR Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with RICTOR siRNA and decrease in signal intensity was observed in Western Blot application using Anti-RICTOR Polyclonal Antibody (Product # MA5-15006). {KD}

RICTOR Antibody — Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western blot using Rictor Monoclonal Antibody (Product # MA5-15006), shows that HCT116 serum starved cells treated with EGF and upon treatment with Curcumin showed a decrease in expression for RICTOR as expected. In addition, there was an increase in the expression of RICTOR in A549 cell lines upon treatment with MG132. {TM}

Immunogen image

RICTOR Monoclonal Antibody (J.644.6)

Immunogen Image RICTOR Monoclonal Antibody (J.644.6)

产品信息

MA5-15006

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

-

产品规格

种属反应

Human, Mouse, Non-human primate, Rat

宿主/亚型

Rabbit / IgG

分类

Monoclonal

类型

Antibody

克隆号

J.644.6

抗原

Synthetic peptide corresponding to the sequence Gln1681 of human Rictor

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

172 µg/mL

17.2 µg

纯化类型

Affinity chromatography

保存液

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

内含物

<0.02% sodium azide

保存条件

-20°C

Ambient (domestic); Wet ice (international)

产品详细信息

It is not recommended to aliquot this antibody.

靶标信息

RICTOR and MTOR (FRAP1) are components of a protein complex that integrates nutrient- and growth factor-derived signals to regulate cell growth.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Cite this product

生物信息学

蛋白别名: AVO3 homolog; DKFZp686B11164; mAVO3; MGC39830; pianissimo; protein pianissimo; TORC2-specific protein AVO3; unnamed protein product

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基因别名: 4921505C17Rik; 6030405M08Rik; AVO3; D530039E11Rik; hAVO3; PIA

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UniProt ID: (Human) Q6R327, (Mouse) Q6QI06

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Entrez Gene ID: (Human) 253260, (Rat) 310131, (Mouse) 78757

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protein serine/threonine kinase activity protein binding ATP binding zinc ion binding protein kinase binding ribosome binding protein serine/threonine kinase activator activity binding, bridging enzyme-substrate adaptor activity

positive regulation of endothelial cell proliferation cytoskeleton organization lipid biosynthetic process embryo development ending in birth or egg hatching actin cytoskeleton organization positive regulation of cell growth positive regulation of cell migration cellular response to nutrient levels TOR signaling positive regulation of TOR signaling regulation of actin cytoskeleton organization TORC2 signaling negative regulation of apoptotic process regulation of protein kinase B signaling positive regulation of protein kinase B signaling regulation of protein phosphorylation regulation of gene expression positive regulation of actin filament polymerization regulation of peptidyl-serine phosphorylation regulation of phosphorylation regulation of inflammatory response positive regulation of peptidyl-tyrosine phosphorylation regulation of establishment of cell polarity protein kinase B signaling cGAS/STING signaling pathway negative regulation of cGAS/STING signaling pathway