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RIP1 Antibody (711689) in ICC/IF
For immunofluorescence analysis, COLO-205 cells were fixed and permeabilized for detection of endogenous RIPK1 using Anti-RIPK1 Recombinant Rabbit Superclonal™ Antibody (Product # 711689, 5 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of RIPK1 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product #... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
711689
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Superclonal
类型
Antibody
抗原
Protein corresponding to human RIPK1 [aa1-aa294]
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
0.5 mg/mL
规格
100 µg
纯化类型
Protein A
保存液
PBS, pH 7.2-7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
产品详细信息
This antibody is predicted to react with Monkey, Horse, Cat
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
靶标信息
Serine-threonine kinase which transduces inflammatory and cell-death signals (programmed necrosis) following death receptors ligation, activation of pathogen recognition receptors (PRRs), and DNA damage. Upon activation of TNFR1 by the TNF-alpha family cytokines, TRADD and TRAF2 are recruited to the receptor. Phosphorylates DAB2IP at 'Ser-728' in a TNF-alpha-dependent manner, and thereby activates the MAP3K5-JNK apoptotic cascade. Ubiquitination by TRAF2 via 'Lys-63'-link chains acts as a critical enhancer of communication with downstream signal transducers in the mitogen-activated protein kinase pathway and the NF-kappa-B pathway, which in turn mediate downstream events including the activation of genes encoding inflammatory molecules. Polyubiquitinated protein binds to IKBKG/NEMO, the regulatory subunit of the IKK complex, a critical event for NF-kappa-B activation. Interaction with other cellular RHIM-containing adapters initiates gene activation and cell death. RIPK1 and RIPK3 association, in particular, forms a necrosis-inducing complex.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: cell death protein RIP; FLJ39204; OTTHUMP00000015955; receptor (TNFRSF)-interacting serine-threonine kinase 1; receptor-interacting protein 1; receptor-interacting protein kinase 1; RIP1 (CT); serine/threonine-protein kinase RIP; unnamed protein product
基因别名: AIEFL; IMD57; RIP; RIP-1; RIP1
UniProt ID: (Human) Q13546
Entrez Gene ID: (Human) 8737
protein kinase activity
protein serine/threonine kinase activity
JUN kinase kinase kinase activity
protein serine/threonine phosphatase activity
death receptor binding
protein binding
ATP binding
ubiquitin protein ligase binding
signaling adaptor activity
identical protein binding
protein homodimerization activity
macromolecular complex binding
death domain binding
protein serine kinase activity
protein serine/threonine kinase binding
MAPK cascade
apoptotic process
inflammatory response
response to oxidative stress
signal transduction
I-kappaB kinase/NF-kappaB signaling
extrinsic apoptotic signaling pathway via death domain receptors
intrinsic apoptotic signaling pathway in response to DNA damage
positive regulation of gene expression
activation of protein kinase activity
positive regulation of interleukin-8 production
positive regulation of tumor necrosis factor production
tumor necrosis factor-mediated signaling pathway
response to tumor necrosis factor
intracellular signal transduction
positive regulation of phosphorylation
positive regulation of apoptotic process
negative regulation of apoptotic process
positive regulation of programmed cell death
positive regulation of I-kappaB kinase/NF-kappaB signaling
negative regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of neuron apoptotic process
positive regulation of macrophage differentiation
positive regulation of transcription from RNA polymerase II promoter
positive regulation of JNK cascade
positive regulation of inflammatory response
regulation of necroptotic process
positive regulation of necroptotic process
negative regulation of necroptotic process
positive regulation of programmed necrotic cell death
positive regulation of interleukin-6-mediated signaling pathway
T cell apoptotic process
necroptotic process
cellular response to hydrogen peroxide
cellular response to tumor necrosis factor
cellular response to growth factor stimulus
extrinsic apoptotic signaling pathway
programmed necrotic cell death
ripoptosome assembly
necroptotic signaling pathway
positive regulation of execution phase of apoptosis
ripoptosome assembly involved in necroptotic process
positive regulation of NIK/NF-kappaB signaling
positive regulation of tumor necrosis factor-mediated signaling pathway
positive regulation of production of miRNAs involved in gene silencing by miRNA
amyloid fibril formation
regulation of reactive oxygen species metabolic process
positive regulation of reactive oxygen species metabolic process
negative regulation of extrinsic apoptotic signaling pathway
positive regulation of extrinsic apoptotic signaling pathway
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
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