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Invitrogen
RNF168 Recombinant Rabbit Monoclonal Antibody (PSH07-37)
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RNF168 Antibody (MA5-60182) in WB
Western blot was performed using RNF168 Recombinant Rabbit Monoclonal Antibody (PSH07-37) (Product # MA5-60182) and 65 kDa band corresponding to RNF168 was observed across cell lines tested. Whole cell extracts (30 µg lysate) of HEK-293 (Lane 1), HeLa (Lane 2), MCF7 (Lane 3), SK-N-MC (Lane 4), Jurkat (Lane 5), K562 (Lane 6) and COLO 205 (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX), 10 well. Resolved proteins were then transferred onto a nitrocellulose membrane by (Product # IB33001) by iBlot™... View More
产品信息
MA5-60182
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
PSH07-37
抗原
Recombinant protein within human RNF168 aa 1-571.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Dry ice (international)
RRID
产品详细信息
Positive Control: K-562 cell lysate, A431 cell lysate, MDA-MB-231 cell lysate, SK-Br-3 cell lysate, HeLa cell lysate, 293T cell lysate, HepG2 cell lysate, U-2 OS cell lysate.
Tissue Specificity: Low tissue specificity.
Subcellular Location: Nucleus.
Sequence Similarities: 65% Mouse/Rat.
Predicted band size: 65 kDa.
靶标信息
RNF168 was identified as a chromatin-associated RING finger protein that acts as a ubiquitin ligase both in vitro and in vivo. RNF168 targets histones H2A and H2AX, but not H2B, forming K63 polyubiquitin chains. Upon formation of DNA double strand breaks, RNF168 is recruited to the site of DNA damage where it co-localizes with -gammaH2AX and 53BP1 in an RNF8-dependent manner. This localization of RNF168 increases the local concentration of ubiquinated proteins to the threshold required for retention of the proteins 53BP1 and BRCA1, facilitating the downstream signaling cascade. Thus, RNF168 defines a new pathway demonstrating a functional cooperation between E3 ligases in genome maintenance. At least three isoforms of RNF168 are known to exist.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: E3 ubiquitin-protein ligase RNF168; FLJ35794; hRNF168; RING finger protein 168; ring finger protein 168, E3 ubiquitin protein ligase; RING-type E3 ubiquitin transferase RNF168
基因别名: hRNF168; RNF168
UniProt ID: (Human) Q8IYW5
Entrez Gene ID: (Human) 165918
double-strand break repair
double-strand break repair via nonhomologous end joining
ubiquitin-dependent protein catabolic process
cellular response to DNA damage stimulus
response to ionizing radiation
protein ubiquitination
protein sumoylation
negative regulation of transcription elongation from RNA polymerase II promoter
histone H2A monoubiquitination
interstrand cross-link repair
histone H2A-K13 ubiquitination
histone H2A-K15 ubiquitination
isotype switching
positive regulation of DNA repair
protein K63-linked ubiquitination
histone H2A K63-linked ubiquitination
