Invitrogen

RNF20 Monoclonal Antibody (3C6E2)

Name: RNF20 Monoclonal Antibody (3C6E2)
Brand: Invitrogen
SKU: MA5-49255

查看其他18个RNF20抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)

图: 1 / 1

RNF20 Antibody (MA5-49255) in WB

Western blot analysis of RNF20 using RNF20 antibody (Product # MA5-49255). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. Lane 1: human Hela whole cell lysates; Lane 2: human MOLT-4 whole cell lysates; Lane 3: human 293T whole cell lysates; Lane 4: human A431 whole cell lysates; Lane 5: rat brain tissue lysates; Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were tran... View More

Product Image Gallery

Immunogen image

Immunogen Image RNF20 Monoclonal Antibody (3C6E2)

产品信息

MA5-49255

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.25-0.5 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Mouse / IgG2b

分类

Monoclonal

类型

Antibody

克隆号

3C6E2

抗原

E.coli-derived human RNF20 recombinant protein (Position: E123-S389).

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 4mg trehalose

内含物

no preservative

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_3074913

产品详细信息

Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

Positive Control - WB: human Hela whole cell, human MOLT-4 whole cell, human 293T whole cell, human A431 whole cell, rat brain tissue, mouse brain tissue.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

靶标信息

RNF20 is an E3 ubiquitin ligase that regulates chromosome structure by monoubiquitinating histone H2B. RNF20 selectively suppresses the expression of some genes such as several protooncogenes and growth-related genes, including many genes that are induced by epidermal growth factor by interfering with chromatin recruitment of transcription elongation factor SII (TFIIS). In contrast, RNF20 also positively regulates the p53 tumor suppressor as well as numerous histone H2A and H2B genes. Together with the deubiquitinase USP44, RNF20 regulates stem cell differentiation by modulating H2B monoubiquitination.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

篇参考文献 (0)

您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。

引用该产品

生物信息学

蛋白别名: BRE1 E3 ubiquitin ligase homolog; BRE1-A; E3 ubiquitin-protein ligase BRE1A; FLJ11189; FLJ20382; HNF3B; homolog of S. cerevisiae BRE1; KIAA2779; MGC129667; MGC129668; RING finger protein 20; RING-type E3 ubiquitin transferase BRE1A; TCF3B

查看更多收起

基因别名: 4833430L21Rik; AW540162; BRE1; BRE1A; C79397; hBRE1; Kiaa4116; mKIAA4116; RNF20

查看更多收起

UniProt ID: (Human) Q5VTR2, (Mouse) Q5DTM8

查看更多收起

Entrez Gene ID: (Human) 56254, (Mouse) 109331, (Rat) 313216

查看更多收起

功能