Invitrogen

ROCK1 Monoclonal Antibody (1H4)

Name: ROCK1 Monoclonal Antibody (1H4)
Brand: Invitrogen
SKU: MA5-17165

查看其他29个ROCK1抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (2)

图: 1 / 2

ROCK1 Antibody (MA5-17165) in WB

Western blot analysis of ROCK1 using ROCK1 monoclonal antibody (Product # MA5-17165) in HEK293 (1) and ROCK1 (AA: 403-610) human IgG Fc transfected HEK293 (2) cell lysate.

Product Image Gallery

产品信息

MA5-17165

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

酶联免疫吸附实验 (ELISA)

1:10,000

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

1H4

抗原

Purified recombinant fragment of human ROCK1 expressed in E. Coli.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2538636

产品详细信息

MA5-17165 targets ROCK1 in indirect ELISA and WB applications and shows reactivity with Human samples.

The MA5-17165 immunogen is purified recombinant fragment of human ROCK1 expressed in E. Coli.

MA5-17165 detects ROCK1 which has a predicted molecular weight of approximately 158kDa.

靶标信息

ROCK1 is a serine/threonine kinase that regulates cell differentiation and migration. The Rho/ROCK pathway is involved in the progression of various human cancers. ROCK1 has been found to be a new Caspase-3 substrate. ROCK, one of the effectors of the small GTPase Rho, has recently been shown to contribute significantly to myosin light chain (MLC) activation through two pathways: direct phosphorylation of MLC and phosphorylation of MLC phosphatase, leading to its inhibition. The increase in cellular contractility that is necessary for apoptotic membrane blebbing implies sustained augmentation of MLC phosphorylation. ROCK-1 consists of an amino-terminal kinase domain and an inhibitory cysteine/histidine-rich C-terminal domain that is located within a pleckstrin-homology region. They are joined by a variable region that contains the Rho-binding domain. During apoptosis, ROCK-I is cleaved by caspase-3 at a conserved DETD1113/G sequence and its carboxy-terminal inhibitory domain is removed, resulting in deregulated and constitutive kinase activity. The caspase-3-mediated cleavage and activation of ROCK I induces phosphorylation of MLC and membrane blebbing, one of the first events in the execution phase of apoptosis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

篇参考文献 (0)

您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。

引用该产品

生物信息学

蛋白别名: MGC131603; MGC43611; p160 ROCK-1; p160-ROCK; p160ROCK; PRO0435; Renal carcinoma antigen NY-REN-35; Rho kinase; Rho-associated protein kinase 1; Rho-associated, coiled-coil containing protein kinase 1; Rho-associated, coiled-coil-containing protein kinase 1; Rho-associated, coiled-coil-containing protein kinase I; ROCK-I

查看更多收起

基因别名: P160ROCK; ROCK-I; ROCK1

查看更多收起

UniProt ID: (Human) Q13464

查看更多收起

Entrez Gene ID: (Human) 6093

查看更多收起

功能

protein kinase activity protein serine/threonine kinase activity protein binding ATP binding GTP-Rho binding metal ion binding non-receptor serine/threonine protein kinase

参与通路