Search
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
ROR gamma (t) Monoclonal Antibody (B2D), eBioscience™
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
图: 1 / 19
ROR gamma (t) Antibody (14-6981-82) in WB
Western blot was performed using Anti-ROR gamma (t) Monoclonal Antibody (B2D), eBioscience™ (Product # 14-6981-82) and a 58 kDa band corresponding to ROR gamma (t) was observed in Mouse Thymus and not in Mouse Spleen, Mouse Testis and Mouse Bone which are reported to be negative. Tissue extracts (60 µg lysate) of Mouse Thymus (Lane 1), Mouse Spleen (Lane 2), Mouse Testis (Lane 3) and Mouse Bone (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitroc... View More
-WB-20170216130641.jpg?time=20220826)
-WB-20170216130641.jpg?time=20220826?time=20220826 "ROR gamma (t) Antibody (14-6981-82) in WB")
产品信息
14-6981-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
已发表种属
C. elegans,
Human,
Mouse
宿主/亚型
Rat
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
B2D
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Description: The B2D monoclonal antibody reacts with the mouse RORgamma(t) protein. RORgamma is a member of the retinoic acid-related orphan receptor (ROR) family, which also includes RORalpha and RORbeta. ROR family proteins are ligand-dependent transcription factors that play roles in multiple physiological processes. RORgamma is expressed in several tissues including liver, lung, muscle, heart and kidney. Furthermore, it was discovered that alternative transcription results in the expression of an isoform, RORgammat, which is expressed exclusively in cells of the lymphoid compartment, namely CD4+CD8+ "double-positive" thymoyctes, Th17 cells of the periphery and lymphoid tissue inducer (Lti) cells of lymphoid organs.
The RORgammat isoform differs from RORgamma by three unique amino acids at the amino terminus. Therefore, the B2D antibody will react with both the RORgamma and RORgammat isoforms.
Applications Reported: This B2D antibody has been reported for use in immunoblotting (WB).
Applications Tested: This B2D antibody has been tested by western blot analysis of mouse thymus nuclear lysates. This B2D antibody can be used at a starting concentration of 2 µg/mL, however the optimal concentration should be determined for individual experimental conditions.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
ROR gamma, a NR1 Thyroid Hormone-Like receptor, has been shown to affect thymopoiesis, bone metabolism, T-cell apoptosis, and lymphoid organogenesis. ROR gamma has been shown to promote thymocyte survival by activating the expression of the antiapoptotic protein Bcl-x(L). ROR gamma is also required for the development of lymph nodes and Peyer's patches. It has been shown that ROR gamma(t), a thymus-specific isoform of ROR gamma from mouse, inhibits Fas ligand expression and cytokine secretion in immature thymocytes. ROR gamma binds as a monomer to response elements composed of a single core motif, GGTCA, preceded by a 6 bp AT-rich sequence. ROR gamma expression has been documented in mouse thymus, adipose, bone, skeletal muscle, liver, and kidney, and in human skeletal muscle. ESTs have been isolated from human tissue libraries, including cancerous head/neck, germ cell, and prostate, and normal colon, head/neck, lung, liver/spleen, and pancreas.
ROR gammat t (RORgt) is an isoform of RORC.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: MGC129539; Nuclear receptor ROR-gamma; Nuclear receptor RZR-gamma; Nuclear receptor subfamily 1 group F member 3; RAR-related orphan receptor C; Retinoid-related orphan receptor-gamma; ROR Gamma; ROR gamma t; RORgt; RP11-98D18.11-001; Thymus orphan receptor; TOR
基因别名: Nr1f3; Rorc; Rorg; RORgamma; Thor; TOR
UniProt ID: (Mouse) P51450
Entrez Gene ID: (Mouse) 19885
transcription coactivator binding
DNA binding
transcription factor activity, sequence-specific DNA binding
steroid hormone receptor activity
RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding
protein binding
oxysterol binding
zinc ion binding
sequence-specific DNA binding
metal ion binding
transcription factor activity, direct ligand regulated sequence-specific DNA binding
C4 zinc finger nuclear receptor
transcription, DNA-templated
regulation of transcription, DNA-templated
protein phosphorylation
xenobiotic metabolic process
multicellular organism development
regulation of glucose metabolic process
regulation of steroid metabolic process
cell differentiation
interleukin-17 production
circadian regulation of gene expression
T cell differentiation in thymus
cellular response to sterol
T-helper cell differentiation
positive regulation of circadian rhythm
steroid hormone mediated signaling pathway
regulation of gamma-delta T cell differentiation
regulation of fat cell differentiation
positive regulation of transcription, DNA-templated
alpha-beta T cell differentiation
rhythmic process
lymph node development
mucosal-associated lymphoid tissue development
Peyer's patch development
adipose tissue development
regulation of transcription involved in cell fate commitment
negative regulation of thymocyte apoptotic process
T-helper 17 cell differentiation
