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Agrisera
RPB1/RNA polymerase II subunit B1 Polyclonal Antibody
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RPB1/RNA polymerase II subunit B1 Antibody (AS11-1804) in ChIP
Chromatin immunoprecipitation (ChIP). Chromatin immunoprecipitation was performed as described (Bowler et al. (2004), Plant Journal) with modifications. Sonic and IP buffer were prepared as described (Kaufmann et al. (2010)), Nature Protocols). Chromatin pellet was sonicated at 4 °C with a Diagenode Bio Ruptor set at high intensity for 10 min (30 sec on, 30 sec off intervals) to obtain 250-500 bp DNA fragme... View More
产品信息
AS11-1804
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated synthetic peptide derived from known higher plant polymerase II sequences.
偶联物
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
RPB1 encodes the largest catalytic subunit of eukaryotic RNA polymerase II (the POLR2A gene product), a predominantly nuclear, chromatin-associated enzyme complex responsible for DNA-templated transcription of protein-coding genes into precursor mRNA. The RPB1 polypeptide forms the core of the polymerase active-center framework and, together with other polymerase subunits, contributes to the DNA-binding cleft that positions the template strand for RNA synthesis. A defining structural feature is its carboxy-terminal domain (CTD), a low-complexity tail composed of tandem heptapeptide repeats with the consensus sequence YSPTSPS; in mammals the CTD comprises 52 repeats and is essential for polymerase activity. Dynamic post-translational modification of CTD residues-especially phosphorylation of Ser2 and Ser5 across the repeats-coordinates transitions through initiation, elongation, and termination and functionally couples transcription to co-transcriptional RNA processing by recruiting and organizing factors required for 5-prime capping, splicing, and 3-prime end cleavage/polyadenylation on nascent transcripts.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: At4g35800; F4B14.70; NRPB1; RNA_POL_II_LS; RNA_POL_II_LSRNA_POL_II_LS; RPB1
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