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Invitrogen
RPL10 Polyclonal Antibody
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RPL10 Antibody (PA5-143918) in ICC/IF
Immunocytochemistry analysis of RPL10 in MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL RPL10 polyclonal antibody (Product # PA5-143918) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the la... View More
产品信息
PA5-143918
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived human RPL10 recombinant protein (Position: R3-S214).
偶联物
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 4mg trehalose
内含物
no preservative
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human K562 whole cell, human HEK293 whole cell, rat C6 whole cell, mouse NIH/3T3 whole cell. IHC: human gall bladder adenosquamous carcinoma tissue, mouse brain tissue, rat brain tissue. ICC/IF: MCF-7 cell. Flow: JK cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
靶标信息
This gene encodes a ribosomal protein that is a component of the 60S ribosome subunit. The related protein in chicken can bind to c-Jun and can repress c-Jun-mediated transcriptional activation. Some studies have detected an association between variation in this gene and autism spectrum disorders, though others do not detect this relationship. There are multiple pseudogenes of this gene dispersed throughout the genome. Alternative splicing results in multiple transcript variants.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 24.6 kDa protein; 24.6kDa protein; 60S ribosomal protein L10; Laminin receptor homolog; Large ribosomal subunit protein uL16; Protein QM; Protein QM homolog; ribosomal protein 10; Ribosomal protein L10; RPLPO; Tumor suppressor QM; Wilms tumor-related protein
基因别名: AUTSX5; D0HXS648; DXHXS648; DXHXS648E; DXS648; DXS648E; L10; NOV; QM; QM16; RPL10
UniProt ID: (Human) P27635, (Rat) Q6PDV7, (Mouse) Q6ZWV3
Entrez Gene ID: (Human) 6134, (Rat) 81764, (Mouse) 110954
