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Agrisera
RPL33/50S ribosomal protein L33 (chloroplastic) Polyclonal Antibody
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RPL33/50S ribosomal protein L33 (chloroplastic) Antibody (AS16-4082) in WB
10 µg of stromal protein from Arabidopsis thaliana (1) and Zea mays (2) denatured at 95°C 10 minutes were separated on 4-20% SDS-PAGE gradient gel and blotted 1 h to PVDF using wet transfer (80 V 1 h 30 minutes). Blot was blocked with 5% milk in TBS-T 1 h/RT. Blot was incubated in the primary antibody at a dilution of 1:2,000 in TBS-T or ON/4°C with agitation. The antibody solution was decanted and the blot... View More
产品信息
AS16-4082
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated peptide, derived from Arabidopsis thaliana RPL33.
偶联物
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana, Hordeum vulgare, Zea mays
靶标信息
RPL33 encodes a small, basic cytosolic ribosomal protein that localizes predominantly to the nucleolus and cytoplasm as a structural constituent of the large (60S) ribosomal subunit, where it associates with rRNA within the mature 80S ribosome and in pre-ribosomal particles during assembly. The encoded protein is part of the eukaryotic L33e family and is typically a short polypeptide enriched in positively charged residues consistent with rRNA binding, with multiple transcript variants reported that generally preserve the same coding sequence. Functionally, RPL33 contributes to ribosome biogenesis and efficient joining of the 40S and 60S subunits during translation initiation, thereby supporting global protein synthesis; perturbation of L33-family proteins has been shown to compromise large-subunit maturation and subunit joining, with downstream effects on translational control.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 50S ribosomal protein L33; 50S ribosomal protein L33 chloroplastic; chloroplast 50S ribosomal protein L33; ribosomal protein L33; rpl33
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