Invitrogen

RUNX1 Recombinant Rat Monoclonal Antibody (RXDMC)

Name: RUNX1 Recombinant Rat Monoclonal Antibody (RXDMC)
Brand: Invitrogen
SKU: 740114T

高级验证

查看其他42个RUNX1抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (3)
高级验证 (2)

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RUNX1 Antibody (740114T) in WB

Western blot was performed using RUNX1 Recombinant Rat Monoclonal Antibody (RXDMC) (Product # 740114T) and ~50 kDa band corresponding to RUNX1 was observed in HEL 92.1.7 and Jurkat as compared to BT-549, T-47D and IMR-32 which is reported to be negative for RUNX1 expression. Nuclear enriched extracts (1% SDS) (30 µg lysate) of HEL 92.1.7 (Lane 1), Jurkat (Lane 2), BT-549 (Lane 3), T-47D (Lane 4) and IMR-32 (Lane 5) were electrophoresed using Novex™ NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transf... View More

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RUNX1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Relative expression of RUNX1 was observed in HEL 92.1.7 and Jurkat when compared to BT-549, T47D and IMR32 which are reported to be negative using RUNX1 Recombinant Rat Monoclonal Antibody (RXDMC) (Product # 740114T) in Western blot. {RE}

产品信息

740114T

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500

流式细胞分析 (Flow)

0.25 µg/test

产品规格

种属反应

Human

宿主/亚型

Rat / IgG2a, kappa

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

RXDMC

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

产品详细信息

Recombinant rat monoclonal antibodies are produced using in vitro expression systems. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity and lot-to-lot consistency.

It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

靶标信息

RUNX1 belongs to the mammalian RUNX family of DNA binding proteins that regulate the expression of genes involved in cellular differentiation and cell cycle progression. RUNX1 is essential for hematopoietic stem cell generation in the vascular regions of the aorta, vitelline and umbilical arteries, yolk sac and placenta. RUNX1 is expressed in all tissues except brain and heart, with highest levels in thymus, bone marrow and peripheral blood.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Acute myeloid leukemia 1 protein; AML-1; aml1 oncogene; AML1-EVI-1 fusion protein; CBF-alpha 2; CBF-alpha-2; Core-binding factor subunit alpha-2; core-binding factor, runt domain, alpha subunit 2; Oncogene AML-1; OTTHUMP00000108697; OTTHUMP00000108700; OTTHUMP00000108702; PEA2-alpha B; PEBP2-alpha B; Polyomavirus enhancer-binding protein 2 alpha B subunit; RUN1; Runt-related transcription factor 1; RUNX1/ZNF687 fusion; SL3-3 enhancer factor 1 alpha B subunit; SL3/AKV core-binding factor alpha B subunit; transcription factor

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基因别名: AML1; AML1-EVI-1; AMLCR1; CBF2alpha; CBFA2; EVI-1; PEBP2aB; PEBP2alpha; RUNX1

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UniProt ID: (Human) Q01196

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Entrez Gene ID: (Human) 861

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功能