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Invitrogen
RUVBL2 Monoclonal Antibody (A1F1)
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RUVBL2 Antibody (MA5-36099) in ICC/IF
Immunocytochemical analysis of RUVBL2 in HT-29 cells using a RUVBL2 monoclonal antibody (Product #MA5-36099). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI.
产品信息
MA5-36099
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Mouse
/ IgG2b
分类
Monoclonal
类型
Antibody
克隆号
A1F1
抗原
Recombinant protein within Human RUVB2 aa 130-321
偶联物
形式
Liquid
浓度
2 mg/mL
纯化类型
Protein G
保存液
PBS, pH 7.4, with 0.2% BSA, 50% glycerol
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
靶标信息
Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (5' to 3') activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL2 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex. May also inhibit the transcriptional activity of ATF2.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 48 kDa TATA box-binding protein-interacting protein; 48 kDa TBP-interacting protein; 51 kDa erythrocyte cytosolic protein; ECP-51; erythrocyte cytosolic protein, 51-KD; INO80 complex subunit J; p47 protein; Repressing pontin 52; reptin; Reptin 52; reptin52 protein; RuvB (E coli homolog)-like 2; RuvB-like 2; RuvB-like protein 2; RUVBL2 protein; TAP54-beta; TIP49b; TIP60-associated protein 54-beta
基因别名: CGI-46; ECP-51; ECP51; INO80J; mp47; p47; REPTIN; RUVBL2; RVB2; TAP54-beta; TIH2; TIP48; TIP49B
UniProt ID: (Human) Q9Y230, (Mouse) Q9WTM5
Entrez Gene ID: (Human) 10856, (Mouse) 20174, (Rat) 292907
RNA polymerase II core promoter sequence-specific DNA binding
RNA polymerase II distal enhancer sequence-specific DNA binding
TFIID-class transcription factor binding
DNA helicase activity
ATP-dependent DNA helicase activity
protein binding
ATP binding
ATPase activity
chromatin DNA binding
identical protein binding
protein homodimerization activity
ATP-dependent 5'-3' DNA helicase activity
ADP binding
unfolded protein binding
ATPase binding
nucleotide binding
damaged DNA binding
helicase activity
hydrolase activity
molecular_function
DNA repair
DNA recombination
chromatin remodeling
transcription, DNA-templated
regulation of transcription, DNA-templated
cellular response to DNA damage stimulus
chromatin modification
DNA duplex unwinding
cellular response to UV
positive regulation of histone acetylation
histone H4 acetylation
histone H2A acetylation
positive regulation of transcription from RNA polymerase II promoter
establishment of protein localization to chromatin
cellular response to estradiol stimulus
transcriptional activation by promoter-enhancer looping
negative regulation of estrogen receptor binding
biological_process
box C/D snoRNP assembly
protein folding
regulation of growth
positive regulation of telomerase RNA localization to Cajal body
