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Invitrogen
Rabbit anti-Goat IgG (H+L) Superclonal™ Secondary Antibody
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Goat IgG (H+L) Secondary Antibody (SA5-10386) in ICC/IF
Immunofluorescence analysis of Rabbit anti-Goat IgG (H+L) Superclonal™ Recombinant Secondary Antibody (Product # SA5-10386) was performed on fixed and permeabilized HeLa cells blocked with SuperBlock™ Blocking Buffer at room temperature. The cells were stained with HSPA9 Polyclonal Antibody (Product # PA5-48035, 1:250) diluted in SuperBlock™ Blocking Buffer (Product # 37515, 1:20) overnight at 4 degrees Cel... View More
产品信息
SA5-10386
应用
建议稀释比
免疫印迹 (WB)
1:1,000
免疫组化（石蜡） (IHC (P))
1:250
免疫细胞化学 (ICC/IF)
1:1,000-1:2,500
酶联免疫吸附实验 (ELISA)
1:1,000
产品规格
种属反应
Goat
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Superclonal
类型
Secondary Antibody
抗原
Purified Goat IgG
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
靶标
IgG
抗体形式
Whole Antibody
产品详细信息
The Rabbit anti-Goat IgG (H+L) Superclonal™ Recombinant Secondary Antibody specifically detects the heavy and light chains of goat IgG. It has ~20% cross reactivity to Hamster IgG and showed minimum to no cross-reactivity to various species immunoglobulins tested.
This recombinant antibody has been engineered with point mutations that eliminate binding to Fc receptors, enabling reduction in background and superior signal to noise.
The sensitivity and specificity of each lot is confirmed using ELISA.
Superclonal™ recombinant secondary antibodies are produced using a multifaceted clonal selection process that involves several phenotypic screens to ensure enhanced specificity, animal origin free formulation and lot-to-lot consistency.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
靶标信息
Anti-Goat secondary antibodies are affinity-purified antibodies with well-characterized specificity for goat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
