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Invitrogen
Rabbit anti-Mouse IgG1 Recombinant Secondary Antibody, Alexa Fluor™ Plus 488
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Mouse IgG1 Secondary Antibody (SA5-10383-AFP488) in ICC/IF
Immunofluorescence analysis of Rabbit anti-Mouse IgG1 Recombinant Secondary Antibody, Alexa Fluor™ Plus 488 (Product # SA5-10383-AFP488) was performed on fixed and permeabilized HeLa cells blocked with SuperBlock™ Blocking Buffer at room temperature. The cells were stained with beta Tubulin Monoclonal Antibody (2 28 33) (Product # 32-2600, 1:500) diluted in SuperBlock™ Blocking Buffer (Product # 37515, 1:20... View More
产品信息
SA5-10383-AFP488
应用
建议稀释比
免疫组化（石蜡） (IHC (P))
1:400
免疫细胞化学 (ICC/IF)
1:1,000-1:2,500
产品规格
种属反应
Mouse
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Secondary Antibody
克隆号
87H11L26
抗原
Purified Mouse IgG
偶联物
激发/发射光谱
493/518 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
proprietary buffer, pH 6.3-7.3
内含物
0.008% Bromonitrodioxane, 0.008% Methylisothiazolone
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Wet ice
靶标
IgG1
抗体形式
Whole Antibody
产品详细信息
The Rabbit anti-Mouse IgG1 Recombinant Secondary Antibody, Alexa Fluor™ Plus 488 is specific to Mouse IgG1 with no reactivity towards Mouse IgG2a, Mouse IgG2b or Mouse IgG3. It showed <10% reactivity to Human IgG, Rat IgG, Goat IgG, Guinea Pig IgG, Hamster IgG , Monkey IgG and Rat IgM.
This recombinant antibody has been engineered with point mutations that eliminate binding to Fc receptors, enabling reduction in background and superior signal to noise.
The sensitivity and specificity of each lot is confirmed using ELISA.
Alexa Fluor™ Plus dyes have been chemically optimized to provide higher signal to noise ratios and minimized cross-reactivity to help detect low-abundance targets in rare or precious samples.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
靶标信息
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
