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Rat IgG (H+L) Secondary Antibody (31834) in WB
Western blot analysis was performed on whole cell extracts (30 µg lysate) of Hep G2 (Lane 1) and MCF7 (Lane 2). The blots were probed with Anti-Alpha Tubulin Rat Monoclonal Antibody (Product # MA1-80017, 1 µg/mL) and detected by chemiluminescence using Rabbit anti-Rat IgG (H+L) Secondary Antibody, Biotin (Product # 31834) at dilutions 1:50,000 (Fig. 1), 1:200,000 (Fig. 2) and 1:400,000 (Fig. 3). A 52 kDa ba... View More
产品信息
31834
应用
建议稀释比
免疫印迹 (WB)
1:50,000-1:400,000
免疫组化 (IHC)
1:500-1:5,000
免疫细胞化学 (ICC/IF)
1:500-1:5,000
酶联免疫吸附实验 (ELISA)
1:200
原位杂交 (ISH)
Assay-dependent
产品规格
种属反应
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Secondary Antibody
偶联物
形式
Liquid
浓度
1.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.8, with 3mg/mL BSA
内含物
0.08% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.
运输条件
Ambient
RRID
AB_228448
靶标
IgG
抗体形式
Whole Antibody
产品详细信息
Product # 31834 has been successfully used in Western blot, IF, ICC, IHC and ISH applications.
Product # 31834 reacts with heavy chains of rat IgG and with the light chains common to most rat immunoglobulins. Based on solid-phase enzyme immunoassay, this antibody cross-reacts with hamster IgG (25%) mouse IgM, rat IgM (12%) bovine IgG, horse IgG, mouse IgG (6%) guinea pig IgG, swine IgG (3%) bovine IgM, cat IgG, dog IgG, dog IgM, goat IgG, sheep IgG (1%). It has minimum cross-reactivity with immunoglobulins from other species.
The activity measured by a solid phase binding assay is 94%.
To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C.
靶标信息
Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
