Search
Agrisera
Ramy 3D/Alpha-amylase isozyme 3D Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
图: 1 / 2
Ramy 3D/Alpha-amylase isozyme 3D Antibody (AS10-716) in WB
25 µg of total protein from Oryza sativa embryos and aleurones (respectively E and A in the figure) were treated with Glucose 100 m M and GA 3 for 24 hr and 48 hr. Then protein were extracted with an SDS Extraction Buffer (60 m M Tris-HCl p H 8.0,2% SDS, 1,5% Sucrose) and were separated on XT CRITERION 10%Bis-Tris (Bio-Rad) SDS-PAGE and blotted 1 h to PVDF. Blot was blocked immediately in milk in TBS-T for ... View More
产品信息
AS10-716
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated synthetic peptide derived from known Oryza sative.
偶联物
形式
Lyophilized
规格
100 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 100 µL of sterile water.
Specific Species Reactivity: Oryza sativa
靶标信息
Ramy3D is a rice (Oryza sativa) nuclear gene encoding an alpha-amylase that enters the secretory pathway and functions extracellularly/apoplastically to hydrolyze internal alpha-1,4-glucosidic bonds in starch and related alpha-glucans, thereby mobilizing carbohydrate reserves to support growth and metabolism when sugars are limiting. The encoded enzyme is a typical plant alpha-amylase polypeptide of ~436 amino acids, produced with an N-terminal signal peptide and a conserved catalytic architecture characteristic of glycoside hydrolase family 13, including the central (beta/alpha)8-barrel catalytic domain and a C-terminal beta-sandwich domain that contribute to substrate binding and enzymatic stability. At the genomic level, RAmy3D is a single member of a multi-gene alpha-amylase family and is physically linked to the related RAmy3E locus; its transcript is detected in multiple tissues (notably germinating seeds and callus) and is strongly regulated by carbon status, being repressed by sugars and induced upon sugar starvation through promoter elements that mediate metabolic control of transcription.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
蛋白别名: alphaAmy3; LOC_Os08g36910; Os08g0473900; OsAmy3D; OsRAM3D; pOS137; RAmy3D
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
