Invitrogen

RbAp46/RbAp48 Monoclonal Antibody (15G12)

RbAp46/RbAp48 Antibody in Immunocytochemistry (ICC/IF)

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RbAp46/RbAp48 Antibody (MA1-23277) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of RbAp46/RbAp48 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: RbAp46/RbAp48 Monoclonal Antibody (15G12) (Product # MA1-23277) diluted at 1:500. Red: phalloidin, a cytoskeleton marker.

RbAp46/RbAp48 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RbAp46/RbAp48 Antibody in Western Blot (WB)

RbAp46/RbAp48 Antibody in Immunoprecipitation (IP)

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产品信息

MA1-23277

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

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免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

免疫沉淀 (IP)

0.2-1 µg/mL

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产品规格

种属反应

Human, Mouse

已发表种属

Human

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

15G12

抗原

The complete coding region (amino acids 1-425) of RbAp48 expressed in E. coli.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS, pH 7, with 20% glycerol

内含物

no preservative

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2177637

产品详细信息

Recommended positive controls: K562, THP-1, HL-60.

Predicted molecular weight is 47.6kDa.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

RbAp48-15G12 recognizes full length RbAp48 (48 kDa) and RbAp46 (46 kDa), related nuclear proteins first isolated by their ability to interact with the carboxyl-terminus of the retinoblastoma protein (Rb). These two proteins are highly homologous at the amino acid level and are related functionally to members of the WD (Trp-Asp) repeat family of proteins. RbAp48 is one of the three subunits of CAF-1, can bind to histone H4 and is a subunit of human histone deacetylase HD1. Both RbAp48 and RbAp46 interact with HDAC1 and mSin3 in vivo.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Alu elements in ANRIL non-coding RNA at chromosome 9p21 modulate atherogenic cell functions through trans-regulation of gene networks.

PLoS genetics

Holdt LM,Hoffmann S,Sass K,Langenberger D,Scholz M,Krohn K,Finstermeier K,Stahringer A,Wilfert W,Beutner F,Gielen S,Schuler G,Gäbel G,Bergert H,Bechmann I,Stadler PF,Thiery J,Teupser D

Published figure using RbAp46/RbAp48 monoclonal antibody (Product # MA1-23277) in Immunoprecipitation

Wed Jan 08 00:00:00 EST 2014

Dual retinoblastoma-binding proteins with properties related to a negative regulator of ras in yeast.

The Journal of biological chemistry

Qian YW,Lee EY

MA1-23277 was used in western blot to characterize RbAp46 and study its functional properties

Fri Oct 27 00:00:00 EDT 1995

生物信息学

蛋白别名: CAF-1 p48 subunit; CAF-1 subunit C; CAF-I 48 kDa subunit; CAF-I p48; Chromatin assembly factor 1 subunit C; Chromatin assembly factor I p48 subunit; chromatin assembly factor/CAF-1 p48 subunit; G1/S transition control protein-binding protein; G1/S transition control protein-binding protein RbAp46; Histone acetyltransferase type B subunit 2; Histone-binding protein RBBP4; Histone-binding protein RBBP7; MSI1 protein homolog; Nucleosome-remodeling factor subunit RBAP46; Nucleosome-remodeling factor subunit RBAP48; RBBP-4; RBBP-7; retinoblastoma binding protein 4; retinoblastoma binding protein 7; Retinoblastoma-binding protein 4; Retinoblastoma-binding protein 7; Retinoblastoma-binding protein p46; Retinoblastoma-binding protein p48; retinoblastoma-binding protein RbAp46

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基因别名: AA409861; AI173248; AU019541; BB114024; lin-53; mRbAp46; mRbAp48; NURF55; RBAP46; RBAP48; RBBP4; RBBP7