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RbcS/Ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit (Algal) Polyclonal Antibody
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RbcS/Ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit (Algal) Antibody (AS22-4825) in WB
Samples: 1-4 Arabidopsis thaliana leaf tissue 5-8 Arabidopsis thaliana soluble extract from leaf tissue 40 mg of fresh Arabidopsis thaliana leaf tissue was used for extraction of the protein. To 40 mg of the tissue was resuspended in 400 µL of extraction buffer. Extraction buffer consists of following components: 100 m M EDTA p H 8.0,120 m M Tris-HCl p H 6.8,4%w/v SDS, 10% v/v ß-Mercaptoethanol, 5% v/v glyc... View More
产品信息
AS22-4825
产品规格
种属反应
Algae,
Bacteria,
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated peptide derived from Chlamydomonas reinhardtii RbcS.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana, Chlamydomonas reinhardtii, Synechococcus sp. PCC7002
靶标信息
RbcS encodes the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) and, in land plants, is typically carried as a nuclear multigene family whose protein product is synthesized in the cytosol as a precursor bearing an N-terminal chloroplast transit peptide that is cleaved after import, yielding a mature small subunit that accumulates in the chloroplast stroma. In Arabidopsis thaliana, representative family members produce a small-chain polypeptide containing the conserved Rubisco small-chain domain, and the nuclear genes share a conserved exon-intron organization (classically two introns within the coding region) consistent with a duplicated, closely related gene family. The mature RbcS polypeptide assembles with the chloroplast-encoded large subunits (RbcL) to form the canonical Form I L8S8 Rubisco holoenzyme (eight large and eight small subunits). Although the catalytic active site resides in RbcL, RbcS is required for proper holoenzyme biogenesis and contributes to enzyme performance by influencing assembly, stability, and kinetic properties (including parameters affecting CO2 fixation versus O2 competition), thereby helping determine Rubisco content and photosynthetic capacity in green tissues.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 5-bisphosphate carboxylase/oxygenase small subunit; RBCS; ribulose bisphosphate carboxylase small chain; ribulose bisphosphate carboxylase small subunit; ribulose-1; Rubisco small subunit
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